Effects of honeysuckle chlorogenic acid on secretory enzymes,lipoxygenase A4,and biochemical indicators in model mice with aluminum induced Alzheimer's disease
Objective It is to explore the effects of chlorogenic acid from honeysuckle on the secretion enzymes,lipoxy-genase A4(LXA4),and blood biochemical indicators in mice with aluminum induced Alzheimer's disease(AD).Meth-ods Chlorogenic acid was extracted from honeysuckle by ultrasound assisted alcohol extraction method.Seventy mice were randomly divided into normal group,model group,and low,medium and high dose groups of honeysuckle chlorogenic acid,with 14 rats in each group.All the mice in each group except for the normal group were given maltol aluminum by intraper-itoneal injection to establish models of aluminum induced AD,continuously injected for 5 days and stopped for 2 days,to-tally poisoned for 8 weeks.Starting from the 5th week of poisoning,the low,medium and high dose groups of honeysuckle chlorogenic acid were given honeysuckle chlorogenic acid solution 40 mg/kg,80 mg/kg and 160 mg/kg by gavage,respec-tively,while the normal group and the model group were fed with an equal volume of distilled water,all once daily,contin-uously gavaged until the end of the 8th week.At the end of the experiment,the learning memory ability of the mice was tested by Y-type water maze,and the number of tests required to reach the learning standard,the number of memory errors in 20 tests and the error rate of the mice were recorded.The brains of mice were taken to determine the contents of β-secre-tase,α-secretase,γ-secretase,LXA4 and acetylcholinesterase(AchE)in the homogenates of brain tissues by ELISA,and their blood was taken to determine the biochemical indexes[blood glucose,total cholesterol(TC),triacylglycerol(TG),high-density lipoprotein cholesterol(HDL-C),total protein,alanine aminotransferase(ALT),urea nitrogen(BUN),cre-atinine(Cr),uric acid(UA)].Results Compared with the normal group,the number of learning tests,number of memo-ry errors,error rate and the contents of β-secretase,γ-secretase and AchE in brain tissue of the mice in the model group were all significantly increased(all P＜0.05),the contents of LXA4 in brain tissue were significantly decreased(all P＜0.05),and the contents of α-secretase did not change significantly(all P＞0.05);compared with the model group,the number of learning tests,number of memory errors,error rate and the contents of β-secretase,γ-secretase and AchE in brain tissue of the mice in each honeysuckle chlorogenic acid group were all significantly decreased,the content of LXA4 in brain tissue were significantly increased in honeysuckle chlorogenic acid high dose group,learned The number of tests,the number of memory errors,the error rate and the content of β-secretase,γ-secretase and AchE in brain tissue were all sig-nificantly reduced(all P＜0.05),the content of LXA4 in brain tissue of the high dose group of honeysuckle chlorogenic acid was significantly increased(P＜0.05),and there was no significant change in the content of α-secretase in brain tis-sue of all groups of honeysuckle chlorogenic acid(all P＞0.05).Compared with the normal group,the levels of blood glu-cose,TC,TG,ALT,BUN,Cr and UA in the model group and the levels of TC,TG and BUN in the low-and medium-dose groups of honeysuckle chlorogenic acid were significantly increased(all P＜0.05),and the level of HDL-C in the model group and the levels of UA in the middle-and high-dose groups of honeysuckle chlorogenic acid were significantly de-creased(all P＜0.05);compared with the model group,the levels of blood glucose,ALT,BUN,UA in each group of ho-neysuckle chlorogenic acid,the levels of TC and Cr in medium and high dose groups of honeysuckle chlorogenic acid,and the level of TG in the high dose group of honeysuckle chlorogenic acid were all significantly lower(all P＜0.05),while the level of HDL-C in the medium and high dose groups of honeysuckle chlorogenic acid and the level of total protein in the high dose group of honeysuckle chlorogenic acid were all significantly higher(all P＜0.05).Conclusion Chlorogenic acid from honeysuckle may improve AD induced by aluminum exposure via regulating related secretory enzymes,LXA4,and va-rious biochemical indicators.
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维普
