Effect of Bixie Fenqing granules on the expressions of mTOR,α-SMA and Col-Ⅰ protein in rats with renal fibrosis induced by unilateral ureteral obstruction
Objective It is to observe the effect of Bixie Fenqing granules on renal fibrosis related proteins mammalian target of rapamycin(mTOR),α-smooth muscle actin(α-SMA)and collagen type Ⅰ(Col-Ⅰ)in unilateral ureteral ob-struction rats,and to explore the protective effect of this medicine on renal fibrosis.Methods Sixty SPF male SD rats were selected,in which 10 rats were randomly selected as blank group,and the remaining 50 rats were treated with unilateral u-reteral obstruction to establish models of renal fibrosis.The successfully modeled rats were randomly divided into model group,losartan group,and high dose,medium dose and low dose groups of Bixie Fenqing granule,with 10 rats in each group.The high dose,medium dose and low dose groups of Bixie Fenqing granule were respectively given Bixie Fenqing granule 13.12 mL/(kg·d),6.56 mL/(kg·d),3.28 mL/(kg·d)by gavage,the losartan group was given losartan 2.62 mL/(kg·d)by gavage,and the blank group and model group were given the same volume of normal saline by ga-vage,all once daily,continuously treated for 4 weeks.At the end of the last gavage,the albumin/urine creatinine ratio(ACR),24-hour urinary protein quantification(24 h-UPQ)and the levels of serum creatinine(SCr)and blood urea nitro-gen(BUN)were detected,the pathological morphology of the kidneys was observed by HE staining and Masson staining,and the expressions of mTOR,α-SMA,and Col-Ⅰ in renal tissues of the rats were detected by immunohistochemistry and Western blot methods.Results Compared with the blank group,the ACR,24 h-UPQ,SCr and BUN of rats in the model group were significantly increased(all P＜0.05),the renal fibrosis was obvious,and the mean optical density values and relative protein expression of mTOR,α-SMA,and Col-Ⅰ in renal tissues were significantly increased(all P＜0.05).Compared with the model group,the ACR,24 h-UPQ,SCr and BUN of rats in the losartan group,and high dose,medium dose groups of Bixie Fenqing granule were significantly decreased(all P＜0.05),the renal fibrosis was improved,and the mean optical density values and relative protein expression of mTOR,α-SMA,and Col-Ⅰin renal tissues were significantly decreased(all P＜0.05).Conclusion Bixie Fenqing granules can attenuate renal fibrosis and protect residual renal func-tion in rats with unilateral ureteral obstruction,and the mechanism may be related to down-regulating the expressions of mTOR,α-SMA,and Col-Ⅰ.
万方数据
维普
