Therapeutic effect of paeoniflorin on radiation enteritis in mice
Objective It is to explore the preventive and therapeutic efficacy of paeoniflorin by peritoneal perfusion on radiation enteritis.Methods Sixty female C57/B6 mice of SPF clean grade were divided into 6 groups after weighting,with 10 mimce in each group.The mice of all groups including radiotherapy(RT)+ normal saline(NS)group(RT + NS group),RT + dexamethasone(DXM)group(RT + DXM group),RT + low-dose paeoniflorin(PF)group(RT + LPF group),RT +medium-dose PF group(RT + MPF group),RT + high-dose PF(RT + HPF group)except for the normal group were treated with a single abdomen irradiation of 10 MeV 18 Gy by linear accelerator to establish models of radiation enteritis.The mice of each group were respectively given NS,1.25 mg/kg DXM,30 mg/kg PF,60 mg/kg PF,and 90 mg/kg PF by peritoneal perfusion at 2 days before RT,the day of RT,1 to 13 days after RT,the infusion volume was 0.1 mL/10 g.The stools consistency,activity and hair condition of the mice were observed,their weight was daily weighed.The mice were dissected on the 2nd,4th,6th,8th,and 14th days after RT,and the intestinal tissues were taken for HE staining and observation;the survival of mice within 14 d after RT in each group was counted.Results The mice in RT+NS group,RT+ DXM group,RT +LPF group gradually had loose stools,slow movement,rough hair,and significant weight loss after RT,and their intestinal mucosal began to be damaged at 2 d after RT.The mice in the RT +MPF group and RT +HPF group had normal defecation,easy movement,smooth hair,small weight loss,which were significantly better than the other RT groups(all P<0.05),and their intestinal tissue damage was delayed and mild.The mice in the normal group,RT +MPF group,and RT + HPF group all survived(100％)within14 dafter RT;2 miceinthe RT +NS group survived(20％),with a median survival time of 5 d;1 mouse in the RT +DXM group survived(10％),with a median survival time of 5.5 d;4 mice in the RT +LPF group survived(40％),with a median survival time of 8.5 d.The difference in survival curves was statistically significant(all P<0.05).Conclusion The linear accelerator for a single abdomen irradiation with 18Gy can successfully induce radiation enteritis model of mice.Intraperitoneal infusion of paeoniflorin can protect the intestinal mucosa,relieve the digestive tract reaction,reduce the degree of weight loss and improve survival rate,the therapeutic effects of the high-dose is better.
万方数据
维普
