Effects of Qinghua Decoction on serum 5-HT and hippocampal pathology in depression rats with syndrome of spleen-stomach dampness heat
Objective It is to study the effect of Qinghua Decoction on serum 5-HT and hippocampal pathology in de-pression rats with syndrome of spleen-stomach dampness heat.Methods Thirty male SPF Rats were selected,in which 5 rats were randomly selected as blank group,and the remaining rats were fed with high-fat and high-sugar diet and stimula-ted with chronic unpredictable mild stress to establish depression model of syndrome of spleen-stomach dampness heat.The successfully modeled rats were randomly divided into model group(n =6),Qinghua Decoction group(n =6),sertraline group(n =6),and Qinghua Decoction +sertraline group(n =7).The Qinghua Decoction group was given Qinghua De-coction 27.5 g/kg by gavage,the sertraline group was given sertraline 5.21 mg/kg by gavage,and the Qinghua Decoction + sertraline group was given Qinghua Decoction 27.5 g/kg +sertraline 5.21 mg/kg by gavage,the model group was given the same volume of normal saline by gavage,all once daily,continuously treated for 28 days.At the end of intragastric admin-istration,the depression status of the rats was evaluated by forced swimming test,their body weight before and after gavage was recorded,the content of 5-HT in serum was detected by ELISA,and the pathological morphology of hippocampus was observed by HE staining.Results The resting time of forced swimming of rats in the model group was significantly longer than that in the blank group(P<0.05),and those in the Qinghua Decoction group,sertraline group,and Qinghua Decoc-tion +sertraline group were significantly shorter than that in the model group(all P<0.05);there was no statistically sig-nificant difference in the body weight of rats among every groups(all P>0.05),but the change of body weight of rats in the Qinghua Decoction group was significantly lower than that in the sertraline group(P<0.05);the difference in the ser-um level of 5-HT of rats was not statistically significant between the model group and blank group(P>0.05),while the serum level of 5-HT in the sertraline group and Qinghua Decoction +sertraline group were significantly higher than that of the model group(both P<0.05),and the Qinghua Decoction +sertraline group was significantly higher than the Qinghua Decoction group(P<0.05).The hippocampal volume of rats in the model group was decreased,the neuronal cells in the CA2-CA3 area were reduced,and the thickness of sulcus gyrus was thinned;the neuronal cells in CA1-CA3 area of hipp-ocampal tissues of rats in each treatment group were tightly arranged,the cellular morphology was not abnormal,and the thickness of sulcus gyrus was thicker than that of rats in the model group;the neuronal cells in CA1-CA3 area of hippocam-pal tissues were tightly and uniformly arranged in Qinghua Decoction group and Qinghua Decoction + sertraline group,which were better than that in the sertraline group.Conclusion Qinghua Decoction can improve the depressive like behavior of rats with syndrome of spleen-stomach dampness heat,and its mechanism may be related to increasing the content of ser-um 5-HT,restoring the morphological changes of hippocampal pathological tissue.
万方数据
维普
