Decoction for upraising Yang and replenishing stomach with Decoction for Invigorating Yang for Recuperation protects kidney in rats with idiopathic membranous nephropathy via up-regulating protein expressions of Nephrin and CD2AP
Objective It is to observe the protective effect of Decoction for upraising Yang and replenishing stomach with De-coction for Invigorating Yang for Recuperation on the kidney of rats with idiopathic membranous nephropathy(IMN)rats and its effect on the expressions of Nephrin and CD2AP proteins in renal tissue.Methods Forty male SD rats were taken,in which 10 rats were randomly selected as the normal group,and the remaining 30 rats were given sheep anti-rat Fx1A ser-um0.4 mL/100 g by one-time tail vein injection to establish models of IMN.The successfully modeled rats were randomly divided into model group,Chinese medicine group and benazepril group,with 10 rats in each group.The Chinese medicine group was given whole formula granule solution of Decoction for upraising Yang and replenishing stomach with Decoction for Invigorating Yang for Recuperation 9.2 g/(kg·d)by gavage,the benazepril group was given benazepril hydrochloride so-lution0.00088 g/(kg·d)bygavage,andthenormalgroupandmodelgroupweregivenequalamountofdistilledwaterby gavage,all once daily,continuously treated for 6 weeks.At the end of 6 weeks of gavage,24 h urine of rats in each group was retained to determine the levels of 24 h urine protein(24-UTP),blood was taken from abdominal aorta to determine the serum levels of albumin(ALB),total cholesterol(TC),creatinine(Cr),and urea nitrogen(BUN),kidney tissues were taken to observe the pathological morphology of kidneys by HE staining and Masson staining,and the expressions of Neph-rin and CD2AP in renal tissue were detected by immunohistochemistry and Western blot.Results Compared with the normal group,the levels of 24-UTP and serum TC of rats in the model group were significantly higher(all P<0.05),and the lev-el of serum ALB was significantly lower(P<0.05);compared with the model group,the levels of 24-UTP and serum TC of rats in the Chinese medicine group and benazepril group were significantly lower(all P<0.05),and the level of serum ALB was significantly higher(all P<0.05);the differences in the levels of serum Cr and BUN were not statistically signif-icant among every groups(all P>0.05).HE staining and Masson staining showed that the glomerular volume of rats in the model group was increased,with significantly thickened basement membrane and a large amount of deposited rephosphin-gosine;compared with the model group,the glomerular volume of rats in the the Chinese medicine group and benazepril group were significantly decreased,and the rephosphingosine deposition in the glomerulus was significantly reduced.The average optical density and relative protein expression of Nephrin and CD2AP in the renal tissues of rats in the model group were significantly lower than those in the normal group(all P<0.05),while the average optical density and relative pro-tein expression of Nephrin and CD2AP in the renal tissues of rats in the Chinese medicine group and benazepril group were significantly higher than those in the model group(all P<0.05).Conclusion Decoction for upraising Yang and replenis-hing stomach with Decoction for Invigorating Yang for Recuperation can protect the kidney of rats with idiopathic membra-nous nephropathy,the mechanism maybe related to up-regulating the expressions of Nephrin and CD2AP proteins.
Decoction for upraising Yang and replenishing stomachDecoction for Invigorating Yang for Recuperationidiopathic membranous nephropathypodocytesNephrinCD2AP
万方数据
维普
