Effect of decoction for dispeling stasis and generating new tissue on fecal bile acid metabolism in mice with mild to moderate ulcerative colitis
Objective It is to observe the effect of decoction for dispeling stasis and generating new tissue on bile acid metabolism in mice with mild to moderate ulcerative colitis(UC),and to explore its mechanism of action in treating UC.Methods Twenty-two cases C57BL/6J mice were taken,in which 5 mice were randomly selected as blank group,and the remaining rats were fed with 4%sodium glucan sulfate solution by free drinking to establish mild to moderate UC.The suc-cessfully modeled mice were randomly divided into model group,group of decoction for dispeling stasis and generating new tissue and mesalazine group,with 5 mice in each group.The group of decoction for dispeling stasis and generating new tis-sue and mesalazine group were given the corresponding drugs by gavage,and the blank group and model group were given distilled water by gavage,all once daily,continuously treated for 14 days.The activity,diet and defecation of mice in each group were observed;the fecal bile acid metabolism of mice was analyzed by targeted metabolomics technology at the end of the last gavage,and the histopathological morphology of colon was observed by HE staining.Results The mice in the model group were depressed,with lost weight,reduced hair,bloody and loose stools,and infiltration of a large number of inflam-matory cells and neutrophils in colonic tissues.The mice in the group of decoction for dispeling stasis and generating new tissue and mesalazine group were active at the end of gavage,their hair,diet and defecation were normal,with a few in-flammatory cells and neutrophils infiltrating in colonic tissue.Compared with the blank group,the contents of ursodeoxy-cholic acid(UDCA),lithocholic acid(LCA),tauro-α-mouse cholic acid(T-α-MCA),taurohyocholate(THCA),glyco-hyocholic acid(GHCA),ursocholic acid(UCA),hyocholic acid(HCA),deoxycholic acid(DCA),α-mouse cholic acid(α-MCA),taurochenodeoxycholic acid(TCDCA),taurodeoxycholic acid(TDCA),glycosaminoglutaric deoxycholic acid(GHDCA),hyodeoxycholic acid(HDCA),glycosaminolithocholic acid(GLCA),taurosulfocholic acid(TCA),and cho-lic acid(CA)were significantly decreased(all P<0.05),the contents of chenodeoxycholic acid(CDCA),β-mouse cho-lic acid(β-MCA),glycosaminolithocholic acid(GCA),dehydrocholic acid(dehydro-CA)were significantly increased in the model group(all P<0.05);compared with the model group,the contents of UDCA,LCA,T-α-MCA,THCA,GH-CA,UCA,α-MCA,HCA,DCA,TCDCA,TDCA,GHDCA,HDCA,GLCA,TCA and CA were significantly increased(all P<0.05),and the contents of CDCA,β-MCA,GCA,and dehydro-CA were significantly decreased in the group of decoction for dispeling stasis and generating new tissue and mesalazine group(all P<0.05).Conclusion The bile acid profile in the feces of mice with mild-to-moderate ulcerative colitis is significantly changed,and decoction for dispeling sta-sis and generating new tissue can alleviate intestinal inflammation and symptoms via regulating the bile acid profile in feces.
ulcerative colitisbile acid metabolismintestinal immunitydecoction for dispeling stasis and generating new tissue
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