Xue-shuan-xin-mai-ning tablet inhibits calcification of vascular smooth muscle cells via regulating the expressions of TGF-β1 and Runx2
Objective It is to investigate whether Xue-shuan-xin-mai-ning tablet inhibits the calcification of vascular smooth muscle cells(VSMCs)by regulating the expressions of transforming growth factor β1(TGF-β1)and Runt-related transcription factor 2(Runx2).Methods The rat VSMCs at logarithmic growth period were taken and divided into 5 groups:the cells in the negative group were cultured with DMEM culture medium;the cells in the calcification group were cultured with β-GP for 24 h to induce VSMCs calcification;the cells in the low,medium and high dose groups of Xue-shuan-xin-mai-ning tablet were firstly cultured with 125 mg/L,250 mg/L and 500 mg/L Xue-shuan-xin-mai-ning tablet for 24 h,and then cultured with β-GP for 24 h.The cell viability of each group was detected by CCK-8,the content of calci-um,activity of alkaline phosphatase(ALP)and content of TGF-β1 in the cells of each group were detected by ELISA,and the expression of Runx2 protein in the cells was detected by Western blot.Results Compared with the negative group,the cell viability of the calcification group was decreased significantly(P＜0.05),and the content of calcium,activity of ALP,content of TGF-β1 and the expression of Runx2 protein in the cells were increased significantly(all P＜0.05);compared with the calcification group,the cell viability of each Xue-shuan-xin-mai-ning tablet group was increased significantly(all P＜0.05),and the content of calcium,activity of ALP,content of TGF-β1 and the expression of Runx2 protein in the cells were decreased significantly(all P＜0.05),and the changes of each index were concentration-dependent.Conclusion Xue-shuan-xin-mai-ning tablet may inhibit the calcification of VSMCs in rats by regulating the expression of TGF-β1 and Runx2.
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