Effect of modify Powder of Rhizome Ligustici and Scorpion on neurogenic inflammation in rats with cough hypersensitivity syndrome
Objective It is to explore the effect of modify Powder of Rhizome Ligustici and Scorpion on neurogenic in-flammation in rats with cough hypersensitivity syndrome.Methods Thirty-six male SD rats were taken,in which 6 rats were randomly selected as normal group,and the remaining rats were used to establish cough hypersensitivity syndrome models by closed-room fumigation method.The successfully modeled rats were randomly divided into 5 groups,with 6 rats in each group.The high,medium and low dose groups of Modify Powder of Rhizome Ligustici and Scorpion were respectively given 12 g/(kg·d),6 g/(kg·d)and 3 g/(kg·d)of Modify Powder of Rhizome Ligustici and Scorpion by gavage,and the codeine phosphate group was given 10 mg/(kg·d)of codeine phosphate by gavage,while the normal group and model group were given the same amount of distilled water by gavage,all once daily,continuously treated for 3 weeks.The cough responses were respectively tested at the end of 1,2 and 3 weeks of gavage,and the times of coughs within 3 min were re-corded;after 3 weeks of gavage,the pathological morphology of lung tissues was observed by HE staining,the Nissl body in cerebral cortical neurons was observed by Nissen staining,and the protein expressions of substance P(SP),calcitonin gene-related peptide(CGRP),transient receptor potential vanilloid 1(TRPV1)in bronchial mucosal tissues and the ex-pressions of interleukin-6(IL-6),interleukin-1β(IL-1β)in cerebral cortex were detected by immunohistochemistry.Re-sults Compared with the normal group,the cough times of rats in the model group were significantly increased(P＜0.05);the degeneration of bronchial mucosal epithelium,disordered arrangement and hyperplasia of smooth muscle layer,and massive inflammatory cell infiltration in tube wall were found;the morphology of neurons was irregular,and the number of Nissl body was reduced;the integral optical density values of protein expressions of SP,CGRP,and TRPV1 in bronchial mucosal tissues,and those of IL-6,and IL-1β in cerebral cortex were significantly increased(all P＜0.05).Compared with the model group,the cough times of rats in the high,medium dose groups of Modify Powder of Rhizome Ligustici and Scorpion were significantly decreased(P＜0.05),the pathologic changes were improved more or less,and the integral op-tical density values of protein expressions of SP,CGRP,and TRPV1 in bronchial mucosal tissues,and those of IL-6,and IL-1β in cerebral cortex were significantly decreased(all P＜0.05).Conclusion Modify Powder of Rhizome Ligustici and Scorpion can significantly attenuate airway epithelial and cortical neuronal lesions and inhibit cough reflex in rats with cough hypersensitivity syndrome,and the mechanism may be related to the inhibition of neurogenic inflammation.
cough hypersensitivity syndromemodify Powder of Rhizome Ligustici and Scorpionneurogenic inflam-mationrat
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