Effect of Decoction of Belamcanda Rhizome and Ephedra on activation of macrophage and regulation of IL-21/STAT3 pathway in rats with bronchial asthma
Objective It is to explore the effect of Decoction of Belamcanda Rhizome and Ephedra on activation of mac-rophage and regulation of IL-21/STAT3 pathway in rats with bronchial asthma.Methods Thirty-six male SD rats were taken,in which 6 rats were randomly selected as the normal group,and the rest of the rats were used to establish bronchial asthma models by ovalbumin sensitization method.The successfully modeled rats were randomly divided into asthma group,low-dose,medium-dose and high-dose groups of Decoction of Belamcanda Rhizome and Ephedra and dexamethasone group,with 6 rats in each group.The rats in the low-dose,medium-dose and high-dose groups of Decoction of Belamcanda Rhizo-me and Ephedra were given 1 g/kg,2 g/kg and 4 g/kg of Decoction of Belamcanda Rhizome and Ephedra(according to raw drug content)by gavage,the rats in the dexamethasone group were given 1 mg/kg of dexamethasone by gavage,and the rats in the normal group and asthma group were gavaged with the same amount of distilled water,all once daily,contin-uously treated for 20 days.At the end of the final gavage,the lung capacity,lung dynamic compliance and peak expiratory flow rate of the rats were detected by a lung function test system,the levels of interferon-γ(IFN-γ),IL-2,IL-4,and IL-13 in alveolar lavage fluid were detected by ELISA,the percentages of eosinophils and neutrophils in alveolar lavage fluid were calculated,the pathological morphology of lung tissues was observed by HE staining,the positive expression of F4/80 protein in lung tissues was detected by immunohistochemistry,and the positive expressions of IL-21,p-STAT3,and STAT3 proteins in lung tissues were detected by Western blot.Results Compared with the normal group,the lung capacity,dy-namic lung compliance,peak expiratory flow rate,the levels of INF-γ and IL-2 in alveolar lavage fluid of rats in the asthma group were significantly decreased(all P＜0.05),the percentages of eosinophils and neutrophils in alveolar lavage fluid,and the levels of IL-4 and IL-13 in lung tissue,and the integral optical density of relative expression of F4/80 protein,rela-tive expressions of IL-21 in lung tissue and P-STAT3/STAT3 were significantly increased(all P＜0.05),inflammatory in-jury of lung tissue was found.Compared with the asthma group,the lung capacity,dynamic lung compliance,peak expira-tory flow rate,the levels of INF-γ and IL-2 in alveolar lavage fluid of rats in each dose group of Decoction of Belamcanda Rhizome and Ephedra and dexamethasone group were significantly increased(all P＜0.05),the percentages of eosinophils and neutrophils in alveolar lavage fluid,and the levels of IL-4 and IL-13 in lung tissue,and the integral optical density of relative expression of F4/80 protein,relative expressions of IL-21 in lung tissue and P-STAT3/STAT3 were significantly decreased(all P＜0.05),the inflammatory injury of lung tissue was significantly improved,and the improvements of these indexes were more significant with the increase of dose of Decoction of Belamcanda Rhizome and Ephedra.Conclusion De-coction of Belamcanda Rhizome and Ephedra can significantly improve lung function,relieve airway inflammation,improve lung tissue lesions in bronchial asthma rats,which may be related to inhibit activation of macrophage and regulate IL-21/STAT3 pathway.
Decoction of Belamcanda Rhizome and Ephedrabronchial asthmamacrophage activationIL-21/STAT3 pathway
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