Effects of special enteral nutrition preparation for tumor containing perilla oil on nutritional status,immune function and tumor growth in mice with lung cancer
Objective It is to investigate the effects of special enteral nutrition preparation for tumor containing perilla oil on nutritional status,immune function and tumor growth in mice with lung cancer.Methods The mice were given NCI-H460 lung cancer cells by subcutaneous inoculation in their right forelimb shoulders to establish lung cancer models.The successfully modeled mice were randomly divided into model group,normal enteral nutrition group,and perilla oil enteral nutrition group,with 10 mice in each group.The normal enteral nutrition group was given normal enteral nutrition prepara-tion by gavage,the perilla oil enteral nutrition group was given special enteral nutrition preparation for tumor containing pe-rilla oil by gavage,the model group was only freely fed and watered,continuously treated for 4 weeks.The body weight and tumor size of the mice were measured every 3 days during the experiment and the tumor volume was calculated.At the end of the experiment,venous blood was taken from the mice to determine the serum levels of nutrition-related indicators[pre-albumin(PAB),total protein(TP),albumin(ALB),total cholesterol(TC),triacylglycerol(TG),low-density lipopro-tein cholesterol(LDL-C),high-density lipoprotein cholesterol(HDL-C)]and immune-related indicators[immunoglobulin A(IgA),IgM,IgE,interleukin-2(IL-2),IL-6,tumor necrosis factor-α(TNF-α),and transforming growth factor-β(TGF-β)].The livers of the mice was taken to detect the contents of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px)and malondialdehyde(MDA)in liver tissue;the tumor was extracted and weighed,the cell cycle in the tumor tissues was detected by flow cytometry,and the apoptosis of the tumor cells was observed by HE staining.Results Compared with the model group and normal enteral nutrition group,the body weight of mice in the perilla oil enteral nutrition group was significantly increased(P＜0.05),the serum levels of PAB,TP,ALB,TC,TG,LDL-C,HDL-C,IgA,IgM,IgE,IL-2,IL-6 and TNF-α,as well as the contents of SOD and GSH-Px in liver tissues were significantly increased(all P＜0.05),and the serum level of TGF-β and content of MDA in liver tissues were significantly reduced(all P＜0.05),tumor volume and weight were significantly reduced(all P＜0.05),cell cycle of tumor tissue was inhibited with tumor cells ap-optosis.Conclusion Special enteral nutrition preparation for tumor containing perilla oil can improve the nutritional status,enhance the immunity,reduce the oxidative stress in liver,and inhibit the growth of subcutaneous tumors in mice with lung cancer.
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NSTL
万方数据
维普
