Decoction for intestinal recovery alleviates acute ulcerative colitis induced by dextran sulfate sodium in mice
Objective It is to observe the effects of decoction for intestinal recovery on symptoms and inflammation of colonic tissue in mice with acute ulcerative colitis(UC)induced by dextran sulfate sodium(DSS).Methods Forty male C57BL/6 mice were randomly divided into blank group,model group,decoction for intestinal recovery group and mesala-zine group,with 10 mice in each group.The mice of all groups except for the blank group were gavaged with 3%DSS for 7 days to establish acute UC models.On the 8th day of the experiment,the decoction for intestinal recovery group was giv-en granule solution of decoction for intestinal recovery 0.85 g/mL by gavage,the mesalazine group was given mesalazine sustained-release granule suspension 0.08 g/mL by gavage,while the normal group and the model group were given an e-qual volume of distilled water by gavage,all once daily,continuously treated for 7 days.During the experiment,the gener-al condition of the mice was recorded daily and their disease activity index(DAI)was calculated.On the 15 th day of the experiment,the length of colon was measured,the pathological morphology of colon tissue was observed and scored by he-matoxylin-eosin staining,and the levels of tumor necrosis factor-α(TNF-α),interleukin-6(IL-6),interleukin-4(IL-4),interleukin-10(IL-10)in colon tissue were detected by enzyme-linked immunosorbent assay(ELISA),the expressions of ZO-1 and CLAUDIN-1 proteins in colon tissue were detected by Western-Blot.Results Compared with the blank group,the colon length of mice in the model group was significantly shorter(P＜0.05),the DAI,colon histopathological score and the levels of TNF-α and IL-6 in colon tissues were significantly higher(all P＜0.05),the levels of IL-4 and IL-10 in colon tissues were significantly lower(all P＜0.05),and the relative expressions of ZO-1 and Claudin-1 proteins in colon tissue were not significantly changed(all P＞0.05).The colon length of mice in the decoction for intestinal recovery group and mesalazine group was significantly longer than that in the model group(all P＜0.05),and the DAI,colon histopathologi-cal score and levels of TNF-α and IL-6 in colon tissue were significantly lower than those in the model group(all P＜0.05),but the differences in the levels of IL-4 and IL-10 in colon tissue and the relative expressions of ZO-1 and Claudin-1 in colon tissue were not statistically significant compared with those in the model group(all P＞0.05),the differences in each index were also not statistically significant between the decoction for intestinal recovery group and mesalazine group(all P＞0.05).Conclusion Decoction for intestinal recovery can regulate the levels of inflammatory factors in intestinal mucosa,thus to improve the symptoms and relieve inflammation of colon tissue in mice with acute ulcerative colitis induced by DSS,its efficacy is equivalent to mesalazine sustained-release granules.
decoction for intestinal recoveryulcerative colitisinflammatory factorsZO-1Claudin-1
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万方数据
维普
