Effects of Yuxianling decoction on expressions of Pannexin1 inflammatory signaling pathway proteins and inflammatory factors in rats with epilepsy caused by pentylenetetrazol
Objective It is to explore the effects of Yuxianling decoction on expressions of Pannexin1 inflammatory sig-naling pathway proteins and inflammatory factors in rats with epilepsy caused by pentylenetetrazol(PTZ).Methods Eighty healthy male SD rats were taken,in which 10 rats were randomly selected as control group and injected intraperitoneally with normal saline,the remaining 70 rats were injected intraperitoneally with PTZ to establish models of epilepsy.40 suc-cessfully modeled rats were randomly divided into model group,Yuxianling decoction group,probenecid group and sodium valproate group,with 10 rats in each group.The Yuxianling decoction group was given Yuxianling decoction3.24 g/(kg·d)by gavage,the probenecid group was given probenecid 13.3 mg/(kg·d)by gavage,the sodium valproate group was given sodium valproate 31.9 mg/(kg·d)by gavage,and the model group and control group were given the same volume of dis-tilled water by gavage,all once daily,continuously treated for 14 days.At the end of the final gavage,the medial hippo-campus of temporal lobe of the rats was taken to detect the protein expressions of PANX1,interleukin-1β(IL-1β),p65 and phosphorylated p65(p-p65)in hippocampal tissues by Western blot;the blood of abdominal aortic artery was taken to detect the serum levels of IL-1β,interleukin-6(IL-6),and nuclear factor-κB(NF-κB)by ELISA.Results The relative expressions of PANX1,IL-1β,p65 and p-p65 proteins in hippocampal tissues of the model group were significantly higher than those of the control group(all P＜0.05);the relative expressions of PANX1 protein in hippocampal tissues of the Yuxianling decoction group,sodium valproate group and probenecid group were significantly lower than that of the model group(all P＜0.05),and the Yuxianling decoction group was lower than the control group and probenecid group(both P＜0.05),there was no significant difference between the Yuxianling decoction group and sodium valproate group(P＞0.05);the relative expressions of IL-1β,p65 and p-p65 proteins in hippocampal tissues of the Yuxianling decoction group and sodium valproate group were significantly lower than those of the model group and probenecid group(all P＜0.05),and the sodium valproate group was significantly lower than the Yuxianling decoction group(P＜0.05).The serum levels of IL-1β,IL-6 and NF-κB in the model group were significantly higher than those in the control group(all P＜0.05);the serum levels of IL-1β in the Yuxianling group and sodium valproate group were significantly lower than those in the model group and probenecid group(all P＜0.05),and there was no statistical significance between the Yuxianling group and so-dium valproate group(P＞0.05);the serum levels of IL-6 and NF-κB in the Yuxianling group were significantly higher than those in the sodium valproate group(all P＜0.05),but the differences were not statistically significant compared with the model group(all P＞0.05).Conclusion Yuxianling decoction can reduce the expression of PANX1 channel in hipp-ocampal tissues and decrease the production of downstream inflammatory factors IL-1β,IL-6,P65 and P-P65 in rats with epilepsy,which may be one of the mechanisms of its antiepileptic action.
NETL
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万方数据
维普
