Parthenolide protects nerves of rats with epilepsy induced by amygdale electrical ignition and its mechanism
Objective It is to investigate the effects of parthenolide(PTL)on the behavior of rats with epilepsy in-duced by amygdale electrical ignition and its mechanism.Methods Forty male SD rats were randomly divided into 4 groups,with 10 rats in each group.The control group was not electrically stimulated,and was given Tween80 by intraper-itoneal injection every day;the model group,the PTL low-dose group and PTL high-dose group were given once of electrical stimulation every day to prepare the models of amygdala electrical ignition epilepsy,the PTL low-dose group and PTL high-dose groups were given 250 μg/kg and 500 μg/kg of PTL by intraperitoneal injections at 1 h before the electrical stimula-tion,respectively,and the model group was given Tween80 by intraperitoneal injection,totally treated for 15 days.The ep-ileptic seizures of rats in each group were observed;the rats were tested by Morris water maze test at 24 h after electrical stimulation finished,and their escape latency and the percentage of crossing the platform quadrant were recorded;then,the contents of malondialdehyde(MDA)and reduced glutathione(GSH)in cerebral tissue were detected,and the ultrastruc-ture of hippocampal CA1 was observed by transmission electron microscopy.Results With the extension of modeling time,the epileptic seizure classification of rats in each epilepsy modeling group were gradually increased,but the epileptic seizure classification of rats in both the low and high dose groups of PTL was significantly lower than that of the model group(both P<0.05),and the high dose group was significantly lower than the low dose group at the same period(all P<0.05).In the water maze test,the escape latency of rats in the model group was significantly longer than that of the control group from day 1 to 4(all P<0.05),and the percentage of crossing the platform quadrant was significantly lower than that of the con-trol group(all P<0.05);the escape latency of rats in both the low and high dosage groups of PTL was significantly shorter than that of the model group(both P<0.05),and the percentage of crossing the platform quadrant was significantly higher than that of the model group(all P<0.05);in the visual platform test,the differences in the escape latency and swimming speed of rats were not statistically significant among each group(all P>0.05).Compared with the control group,the con-tent of MDA in cerebral tissue of rats were significantly increased while the content of GSH was significantly decreased in the model group(both P<0.05);the hippocampal synaptic gap was significantly widened(P<0.05),the length of the active band was significantly shortened(P<0.05),and the postsynaptic density was significantly reduced(P<0.05).Compared with the model group,the contents of MDA in cerebral tissue of rats were significantly decreased while the con-tents of GSH were significantly increased in both the low and high dose groups of PTL(all P<0.05);the hippocampal synaptic gaps were significantly reduced(both P<0.05),the lengths of the active band were significantly lengthened(both P<0.05),and the postsynaptic densities were significantly increased(both P<0.05).The changes of MDA and GSH contents in cerebral tissues of rats in the PTL high-dose group were more than those in the PTL low-dose group(both P<0.05).Conclusion Parthenolide has a good neuroprotective effect on amygdala epilepsy rats,and its mechanism may be related to its antioxidant effect and neuroprotection of hippocampus ultrastructure.
epilepsyparthenolideoxidative stressultrastructure of hippocampus
NETL
NSTL
万方数据
