Decoction for nourishing Yin and invigorating kidney improves bone metabolism of diabetic rats complicated with ovariectomized osteoporosis through PI3K/Akt/NF-κB signaling pathway
Objective It is to explore the effects of decoction for nourishing Yin and invigorating kidney on bone metab-olism in diabetic rats complicating with ovariectomized osteoporosis rats through phosphatidyl inositol 3-kinase/protein ki-nase B/nuclear factor-kappaB(PI3K/Akt/NF-κB)signaling pathway.Methods Sixty SPF-grade female rats were randomly divided into control group,model group,calcitriol group,and high,medium,and low dose groups of decoction for nouris-hing Yin and invigorating kidney,with 10 rats in each group.The rats in all groups except for the control group were trea-ted with bilateral oophorectomy,fed with high-sugar and high-fat diets,and were injected with streptozotocin to establish models of diabetes mellitus complicating with ovariectomized osteoporosis.After successful modeling,the high,medium and low dose groups of decoction for nourishing Yin and invigorating kidney were given 20 g/kg,10 g/kg and 5 g/kg of med-icated solution of decoction for nourishing Yin and invigorating kidney,respectively,the calcitriol group was given0.1 μg/kg of calcitriol by gavage,and the control group and model group were given an equal volume of normal saline,all once daily,continuously treated for 8 weeks.At the end of drug administration,the blood was collected from femoral artery to deter-mine the levels of fasting plasma glucose(FPG),calcium,phosphorus,triacylglycerol(TG),and cholesterol(TC)by automatic biochemical analyzer,the blood was collected from abdominal aorta to determine the levels of glycated hemoglobin(HbA1c)and fasting insulin(FINS)by ELISA,and the insulin resistance index(HOMA-IR)was calculated;the relative expressions of PI3K,Akt,and NF-κB mRNAs in bone tissues were detected by RT-PCR.Results Compared with the con-trol group,the levels of FPG,HbA1c,HOMA-IR,TG,TC and the relative expression of NF-κB mRNA in bone tissues were significantly increased in the model group(all P<0.05),and the levels of FINS,blood calcium,blood phosphorus and the relative expressions of PI3K mRNA and Akt mRNA in bone tissues were significantly decreased(all P<0.05).Compared with the model group,the levels of FPG,HbA1c,HOMA-IR,TG,TC and the relative expression of NF-κB mRNA in bone tissues were significantly decreased in the calcitriol group and each groups of decoction for nourishing Yin and invigorating kidney(all P<0.05),and the levels of FINS,blood calcium,blood phosphorus and the relative expres-sions of PI3K mRNA and Akt mRNA in bone tissues were significantly increased(all P<0.05),and the improvement of every index in the high dose groups of decoction for nourishing Yin and invigorating kidney was significantly better than that in the low dose group(all P<0.05).Conclusion Decoction for nourishing Yin and invigorating kidney can improve bone metabolism as well as regulate sugar metabolism and relieve insulin resistance through mediating the PI3K/AKT/NF-κB sig-naling pathway in diabetic rats complicating with ovariectomized osteoporosis.
diabetes mellitusosteoporosis decoction for nourishing Yin and invigorating kidneyPI3K/Akt/NF-κB signaling pathways
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