Effect of Diyuqibai Decoction on intestinal microflora in ulcerative colitis rats based on 16S rDNA sequencing
Objective It is to observe the effect of Diyuqibai Decoction on intestinal flora of rats with ulcerative colitis(UC)of damp-heat type and explore its mechanism in the treatment of UC.Methods Eighty-four Wistar rats were taken,in which 9 rats were randomly selected as the blank group,and the rest of the rats were used to establish the models of damp-heat UC by the method of high-sugar and high-fat diet plus dextran sodium sulfate.The successfully modeled rats were randomly divided into model group,sulfasalazine(SASP)group and low,medium and high dose groups of Diyuqibai Decoction,with 14 rats in each group.The SASP group was given SASP suspension 300 mg/kg by enema,the low,medi-um and high dose groups of Diyuqibai Decoction were given medicated serum containing 0.5 g/mL,1 g/mL and 3 g/mL of Diyuqibai Decoction by retained enema,respectively,while the model group and blank group were given equal amounts of normal saline by enema,all once daily,continuously treated for 14 days.The general condition of the rats in each group was observed and scored by disease activity index(DAI),the histopathological changes of their colon tissue were observed by HE staining,the fecal flora species composition was analyzed by 16S rDNA sequencing.Results Compared with the model group,the symptoms such as diarrhea and blood stool of rats in each treatment group were significantly improved,their DAI score were significantly reduced(all P<0.05),and the histopathological damage of colon tissue was significant-ly alleviated.As for the composition of bacterial flora,the abundance of Lachnospiraceae and Mucispirillum in rats of the model group were significantly lower than those in the blank group(all P<0.05),and the abundances in each treatment group were significantly higher than those in the model group(all P<0.05);the abundance of Enterobacteriaceae in the model group was significantly higher than that in the blank group(P<0.05),and the abundance in each treatment group was significantly higher than that in the mdel group(P<0.05).Conclusion Diyuqibai Decoction can significantly reduce disease activity and improve intestinal mucosal inflammatory injury in rats with damp-heat ulcerative colitis.Its mechanism may be related to regulate intestinal flora,increase the abundance of beneficial bacteria and reduce the abundance of patho-genic bacteria.
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