Shensong Yangxin Capsule plays a protective role in myocardial ischemia-reperfusion injury through iron death pathway
Objective It is to explore the protective effect and its internal mechanism of Shensong Yangxin Capsule on myocardial ischemia-reperfusion injury.Methods H9c2 cells were randomly divided into control group,glyoxy-deprivation/reoxygenation(OGD/R)group,OGD/R+low dose ShensongXinjiang group,OGD/R+medium dose Shensongxinjiang group and OGD/R+high dose Shensongxinjiang group.The H9c2 cells were normal cultured in the control group,and the cells in the other groups were modeled with OGD/R to simulate ischemia-reperfusion injury in cardiomyocytes.The OGD/R+low dose ShensongXinjiang group,OGD/R+medium dose Shensongxinjiang group and OGD/R+high dose Shens-ongxinjiang group were respectively cultured with 0.25 μg/mL,0.5 μg/mL,1.0 μg/mL Shensong Yangxin capsule solu-tion before modeling.The cell survival rate was detected by MTT assay,the contents of reactive oxygen species(ROS),malondialdehyde(MDA),superoxide dismutase(SOD)and Fe2+in the cells were detected by kits,the expression of lipid peroxidation-related factors[acyl-CoA synthetase long-chain family member 4(ACSL4),glutathione peroxidase 4(GPX4)]and iron-death pathway-related proteins[transferrin receptor 1(TfR1),hypoxia-inducible factor-1α(HIF-1α),ferritin heavy chain 1(FTH1),nuclear receptor coactivator 4(NCOA4),heme oxygenase-1(HO-1)]were detec-ted by Western blot assay.Results Compared with the control group,the survival rate,the content of SOD and relative ex-pressions of GPX4,FTH1,and NCOA4 proteins in the cells of OGD/R group were significantly decreased(all P<0.05),while the contents of ROS,MDA,and Fe2+and relative expressions of ACSL4,TfR1,HIF-1α,and HO-1 proteins in the cells were significantly increased(all P<0.05).Compared with the OGD/R group,the survival rate,the content of SOD and relative expressions of GPX4,FTH1,and NCOA4 proteins in the cells of each OGD/R+Shensongxinjiang group were significantly increased(all P<0.05),while the contents of ROS,MDA,and Fe2+and relative expressions of ACSL4,TfR1,HIF-1α,and HO-1 proteins in the cells were significantly decreased(all P<0.05).Conclusion Shensong Yangxin Capsule can alleviate oxidative stress and protect ischemia-reperfusion injured cardiomyocytes via regulating iron death pathway.
NETL
NSTL
万方数据
