Effect of decoction for clearing and resolving on serum dopamine and small intestinal pathological changes in rats with depression of type of dampness and heat
Objective It is to explore the effects of decoction for clearing and resolving on serum dopamine and small intestinal pathology in rats with depression of type of dampness and heat.Methods Thirty SD male rats were taken,in which 5 rats were randomly selected as the blank group,and the remaining 25 rats were fed with high-fat and high-sugar di-et and stimulated with chronic unpredictable mild stress to establish models of spleen-stomach dampness-heat depression.The successfully modeled 25 rats were randomly divided into a model group of 6 rats,a decoction for clearing and resolving group of 6 rats,a sertraline group of 6 rats,and a decoction for clearing and resolving plus sertraline group of 7 rats.The decoction for clearing and resolving group was given decoction for clearing and resolving 27.5 g/(kg·d)by gavage,the sertraline group was given sertraline tablets 5.21 mg/(kg·d)by gavage,and the decoction for clearing and resolving plus sertraline group was given decoction for clearing and resolving 27.5 g/(kg·d)and sertraline tablets 5.21 mg/(kg·d)by gavage,the blank group and model group were given equal volumes of normal saline by gavage,all once daily,continuously traeted for 21 days.The actions of the rats were detected by open field test at the end of the final gavage,and after the test,the serum of the rats was taken to detect the level of dopamine,and their small intestinal tissues were taken to observe the pathology by HE staining.Results The times of crossing the center of the rats of decoction for clearing and resolving group and decoction for clearing and resolving plus sertraline group were significantly more than that of the model group(both P＜0.05),but there was no significant difference in the times of crossing the center among each treatment group(all P＞0.05);the times of crossing the peripheral of the decoction for clearing and resolving plus sertraline group were significant-ly less than those of the blank group and decoction for clearing and resolving group(both P＜0.05);the times of crossing the peripheral of rats of the decoction for clearing and resolving group had a tendency to be more than that of the model group and sertraline group,and was close to that of the blank group,but the differences were not statistically significant when comparing among the 4 groups(all P＞0.05);there was no significant difference in the times of upstanding and grooming of rats among each group(all P＞0.05).There was no significant difference in the serum level of dopamine be-tween the model group and blank group(P＞0.05),the serum level of dopamine of each treatment group was significantly higher than that of the model group(all P＜0.05),and the serum level of dopamine of the sertraline group and decoction for clearing and resolving plus sertraline group were significantly higher than that of the blank group(both P＜0.05).Compared with the blank group,there were inflammatory cells in epithelium and lamina propria of the small intestine with lymph node formation in the lamina propria in rats of the model group,the inflammatory cells in epithelium and lamina pro-pria of the small intestine were reduced in each treatment group compared with the model group,and the improvement was more significant in the decoction for clearing and resolving group compared with other two groups.Conclusion Decoction for clearing and resolving group can improve depressive symptoms,increase serum dopamine concentration in rats with depres-sion of type of dampness and heat,it can protect the small intestine mucosa.
depressiondecoction for clearing and resolvingdamp heat typedopaminesmall intestine
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