Correlation between different traditional Chinese medicine syndrome types and peripheral blood macrophage phenotype in non-alcoholic fatty liver disease
Objective It is to investigate the correlation between traditional Chinese medicine syndrome types and M1 and M2 phenotypes of peripheral blood macrophages in patients with nonalcoholic fatty liver disease(NAFLD).Methods One hundred and forty patients who were treated at the outpatient clinic of the Second Department of Liver,Gallbladder,Spleen and Stomach of Heilongjiang Provincial Hospital of Traditional Chinese Medicine from December 2021 to December 2022 and were clinically diagnosed with NAFLD were selected,and were divided into internal dampness and turbidity reten-tion group(n=27),liver depressions and spleen deficiency group(n=40),dampness-heat accumulation group(n=32),spleen-kidney deficiency group(n=13),phlegm and stasis binding group(n=28).Another 15 healthy subjects were recruited from the hospital's health checkup center as the healthy subject group.The liver function indexes[serum glutamate transpeptidase(ALT),glutamate transpeptidase(AST),gamma-glutamyl transpeptidase(GGT),alkaline phosphatase(ALP)],lipid indexes[triacylglycerol(TG),total cholesterol(TC),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C)],serum inflammation factor indexes[interleukin-6(IL-6),interleukin-8(IL-8),interleukin-10(IL-10),and interleukin-4(IL-4)],macrophage phenotypes,hepatic controlled at-tenuation parameter(CAP)of subjects of each group were detected,and the correlation between macrophage phenotypes and NAFLD disease states in the patients of each group were analyzed by Pearson correlation analysis.Results Compared with the healthy subject group,the serum levels of ALT,AST,GGT,ALP,TC,TG,LDL-C,IL-6,IL-8 levels and the percentages of M1-type macrophages,M1/M2 ratio,and CAP were significantly increased,while the serum levels of HDL-C,IL-10,IL-4 and the percentages of M2-type macrophages were significantly decreased in the patients of each syndrome type group(all P<0.05).The serum levels of ALT,AST,GGT,ALP,TG,IL-6,IL-8 and percentage of M1-type mac-rophages and CAP of patients with different syndrome types from low to high were as follows,liver depressions and spleen deficiency group,spleen-kidney deficiency group,internal dampness and turbidity retention group,phlegm and stasis bind-ing group,dampness-heat accumulation group,and the serum levels of HDL-C,IL-10,IL-4 and percentage of M2-type macrophages from high to low were as follows,liver depressions and spleen deficiency group,spleen-kidney deficiency group,internal dampness and turbidity retention group,phlegm and stasis binding group,dampness-heat accumulation group,and the serum levels of TC and LDL-C from low to high were as follows,liver depressions and spleen deficiency group,internal dampness and turbidity retention group,dampness-heat accumulation group,spleen-kidney deficiency group and phlegm and stasis binding group.Correlation analysis revealed that the serum levels of ALT,AST,GGT,ALP,TC,TG,LDL-C,IL-6,IL-8 and CAP were positively correlated with the percentage of M1-type macrophages(all P<0.05),and negatively correlated with the percentage of M2-type macrophages(all P<0.05);the serum levels of HDL-C,IL-10,IL-4 were negatively correlated with the percentage of M1-type macrophages(all P<0.05),and positively correlated with the percentage of M2 macrophages(all P<0.05).Conclusion Among the Chinese medicine syndrome types of NAFLD,the highest percentage of M1-type macrophages was found in patients with syndrome of damp-heat accumulation,the lowest percentage of M1-type macrophages was found in patients with syndrome of liver-depression and spleen-deficiency,and the rule of the percentage of M2-type macrophages was opposite to that of M1-type macrophages,which suggests that the macro-phage polarization is involved in the occurrence and development of NAFLD.
non-alcoholic fatty liver diseasetraditional Chinese medicine syndrome typemacrophage phenotypecorrelation analysis
万方数据
维普
