Metabolomics of nasal mucosa in patients with chronic rhinosinusitis of syndrome of heat stagnation in gallbladder
Objective It is to characterize the metabolic profile in the nasal mucosa of patients with chronic rhinosinus-itis(CRS)of syndrome of heat stagnation in gallbladder with metabolomics technology.Methods Twenty patients with CRS of syndrome of heat stagnation in gallbladder treated in the outpatient clinic of the Department of Otorhinolaryngology of the First Affiliated Hospital of Heilongjiang University of Chinese Medicine from April 2019 to February 2022 were selected as the CRS group,and 20 healthy volunteers recruited during the same period as the normal group.The nasal mucosal samples from the subjects of the two groups were collected,and the nasal mucosal metabolites were detected by ultra-high perform-ance liquid chromatography mass spectrometry technology.The obtained data matrix was imported into EZinfo 3.0 software for multivariate statistical analysis,the contribution value(VIP)of each ion was determined according to OPLS-DA analy-sis,and the metabolites were screened to find differential metabolites and analyze their metabolic pathways.Results The differential metabolites in the nasal mucosa of subjects of the two groups were mainly manifested as changes in 28 metabo-lites(VIP>1,P<0.05),of which 24 belonged to lipids and lipid-like molecules.The differential metabolites of the two groups were enriched in 4 metabolic pathways,those were ether lipid metabolism,propionic acid metabolism,glycerophos-pholipid metabolism,unsaturated fatty acid biosynthetic pathways.Conclusion Compared with the normal people,the differences in nasal mucosal metabolites in patients with chronic rhinosinusitis of syndrome of heat stagnation in gallbladder are mainly lipids and lipid-like molecules,which suggests that its pathogenesis may be related to abnormal lipid metabo-lism.
chronic rhinosinusitis,metabolomics,liquid chromatography-mass spectrometrysyndrome of heat stagna-tion in gallbladder
万方数据
维普
