Establishment and preliminary identification of models of mouse vitiligo disease
Objective It is to compare the effects of hydroquinone cream and monobenzone cream in inducing vitiligo like phenotype in wild-type C57BL/6J mice,and to construct cost-effective and easy to operate vitiligo like mouse models with high histopathological similarity and long-lasting decolorization effect.This provides a basis for selecting vitiligo like animal models for subsequent research on the pathogenesis of human vitiligo disease,as well as the therapeutic effect and drug action mechanism of drugs on this disease.Methods Thirty C57BL/6J mice were randomly divided into 3 groups,with 10 mice in each group.After the mice of the three groups were depilated on the same parts of back,the control group was applied with cream base,the monobenzone group was applied with 40％monobenzone cream,and the hydroquinone group was applied with 2.5％hydroquinone cream,all once daily,modeled for 50 days.The skin changes at the modeling site were observed with naked eyes;at the end of modeling,the mice in each group were executed,and their skin tissues were taken from the lesion site to detect the expression of tyrosinase(TYR)by immunofluorescence staining;the blood was col-lected from their eyeballs to determine the serum levels of TYR,interferon-γ(IFN-γ),tumor necrosis factor-α(TNF-α),and matrix metalloproteinase-9(MMP-9)by ELISA.Results The mice in both the monobenzone group and hydroquinone group had significant skin discoloration,and their mean optical density value of TYR expression was significantly reduced.The serum levels of TYR of mice in the monobenzone group and hydroquinone group were significantly lower than that in the control group(both P＜0.05),and the difference between the monobenzone group and hydroquinone group was not statisti-cally significant(P＞0.05);the serum levels of IFN-γ and MMP-9 of mice in the hydroquinone group were significantly higher than those of mice in the control group and monobenzone group(all P＜0.05),and the difference in the serum level of TNF-α was not statistically significant between the monobenzone group and control group(P＞0.05);the serum level of TNF-α in the monobenzone group was significantly higher than those in the control group and hydroquinone group(both P＜0.05),and the differences in serum levels of IFN-γ and MMP-9 were not statistically significant when compared with the control group(both P＞0.05).Conclusion Both 2.5％hydroquinone cream and 40％monobenzone cream application methods can successfully establish vitiligo models in wild-type C57BL/6J mice,2.5％hydroquinone cream is more easily to induce vitiligo phenotype.
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