MEBT/MEBO promotes the healing of chronic refractory wounds via Wnt/β-catenin signaling pathway
Objective It is to investigate the mechanism of moist exposed burn therapy/moist exposed burn ointment(MEBT/MEBO)in promoting the healing of chronic refractory wounds via influencing the expression of glycogen synthase kinase-3β(GSK-3β)and Wnt3a in the Wnt/β-catenin signaling pathway.Methods Sixty Wistar male rats were randomly divided into blank group,acute wound group,chronic refractory wound group,MEBT/MEBO group,and rb-bFGF group,with 12 rats in each group.The rats in the blank group were not subjected to any injurious treatment;the rats of the rest group were subjected to total skin excision at a uniform site to create wounds,in which the rats in the acute wound group were not injected with hydrocortisone,and the rats in the other groups were injected with hydrocortisone acetate to create chronic refractory wounds.Then,the wound was cleaned and changed,twice per day,two layers of wound-sized MEBO gauze and rb-bFGF-impregnated gauze were applied to the wound in the MEBT/MEBO group and rb-bFGF group respec-tively,two layers of wound-sized saline gauze were applied to the wound in the rest groups,and two layers of sterilized dry gauze were finally applied on the surface.The healing rate of wounds on the 3rd,7th and 14th day of drug exchange was calculated in each group,and the mRNA and protein expressions of GSK-3β and Wnt3a in the wound tissue were respec-tively detected by RT-PCR and Western blot on the 3rd,7th and 14th day of drug exchange in each group,and the positive expressions of GSK-3β and Wnt3a in the wound tissue were detected by immunohistochemical staining on the 14th day of drug exchange in the chronic refractory wound group and MEBT/MEBO group.Results The wound healing rates at each time point in the acute woud group,MEBT/MEBO group and rb-bFGF group were significantly higher than those in the chronic refractory wound group(all P＜0.05);the wound healing rates at the 7th day of dressing change in the MEBT/MEBO group and rb-bFGF group,and the wound healing rate at the 14th day of dressing change in the MEBT/MEBO group were significantly higher than those in the acute wound group(all P＜0.05).On the 3rd day of dressing change,the rela-tive expressions of GSK-3β mRNA and protein in the wound tissue of the MEBT/MEBO group and rb-bFGF group were sig-nificantly lower than those of the chronic refractory wound group(all P＜0.05);On the 7th day of dressing change,the relative expressions of GSK-3β mRNA and protein in the MEBT/MEBO group and rb-bFGF group were not statistically dif-ferent from those in the chronic refractory wound group(all P＞0.05);on the 14th day of dressing change,the relative ex-pressions of GSK-3β mRNA and protein in the MEBT/MEBO group and rb-bFGF group were significantly higher than those in the chronic refractory wound group(all P＜0.05).On the 3rd and 7th days of dressing change,the relative expressions of Wnt3a mRNA and protein in the MEBT/MEBO group and rb-bFGF group were significantly higher than those in the chronic refractory wound group(all P＜0.05);on the 14th day of dressing change,the relative expressions of Wnt3a mR-NA and protein in the MEBT/MEBO group and rb-bFGF group were significantly lower than those in the chronic refractory wound group(all P＜0.05).On the 14th day of drug exchange,the cell morphology of MEBT/MEBO group was more complete,with obvious neovascularization,the distribution of GSK-3β protein expression was more than that of the chronic refractory wound group,and the distribution of Wnt3a protein expression was less than that of the chronic refractroy wound group.Conclusion MEBT/MEBO may participate in regulating the expressions of Wnt3a and GSK-3β in the Wnt/β-cate-nin signaling pathway to promote wound healing.
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