Effects of vitexicarpin on proliferation,migration and invasion of colorectal cancer cells via UBA2/PTEN/PI3K/Akt pathway
Objective It is to explore the effects of vitexicarpin on the proliferation,migration and invasion of colorectal cancer SW480 cells based on the ubiquitin-like modifier activating enzyme 2(UBA2)/phosphate and tension homology de-leted on chromosome ten(PTEN)/phosphate idylinositol 3-kinase(PI3K)/protein kinase B(Akt)pathway.Methods SW480 cells in logarithmic growth phase were taken and randomly divided into 4 groups.The cells in the control group were routinely cultured,the cells in the vitexicarpin group were cultured with 10 µmol/L vitexicarpin,the cells in the UBA2 in-hibitor group were cultured with 0.5 μmol/L UBA2 inhibitor,and the cells in the vitexicarpin+UBA2 inhibitor group were co-cultured with 10 μmol/L vitexicarpin and 0.5 μmol/L UBA2 inhibitor.The cell proliferation was detected by CCK-8 as-say,the monoclonal formation ability of the cells was observed by clone formation assay,the migration ability of the cells was observed by scratch assay,the invasion ability of the cells was observed by Transwell assay,and the expressions of pro-teins related to UBA2/PTEN/PI3K/Akt pathway in the cells were detected by Western blot.Results CCK-8 assay and clone formation assay showed that the cell proliferation absorbance OD values of the UBA2 inhibitor group and the vitexi-carpin+UBA2 inhibitor group were significantly lower,while the numbers of cell clone formation were significantly less than those of the vitexicarpin group(all P＜0.05),and the differences in the cell proliferation absorbance OD values and number of cell clone formation were not statistically significant between the UBA2 inhibitor group and vitexicarpin+UBA2 inhibitor group at different times of culture(all P＞0.05).Scratch and Transwell experiments showed that the scratch spacings in the UBA2 inhibitor group and vitexicarpin+UBA2 inhibitor group were significantly wider,while the numbers of membrane-penetrating cells were significantly less than those in the vitexicarpin group(both P＜0.05),and the differ-ences were not statistically significant between the UBA2 inhibitor group and vitexicarpi+UBA2 inhibitor group(all P＞0.05).The relative protein expressions of PTEN in the cells of vitexicarpin group,UBA2 inhibitor group and vitexicarpin+UBA2 inhibitor group were all significantly higher than that of the control group(all P＜0.05),and the relative protein ex-pressions of UBA2,p-PI3K,p-Akt were all significantly lower than those of the control group(all P＜0.05);the relative protein expressions of PTEN in the cells of UBA2 inhibitor group and vitexicarpin+UBA2 inhibitor group were all signifi-cantly higher than that of the vitexicarpin group(all P＜0.05),and the relative protein expressions of UBA2,p-PI3K,p-Akt were all significantly lower than those of the vitexicarpin group(all P＜0.05);the differences in the relative protein expressions of UBA2,PTEN,p-PI3K,and p-Akt in the cells were not statistically significant between the UBA2 inhibitor group and vitexicarpin+UBA2 inhibitor group(all P＜0.05).Conclusion Vitexicarpin may inhibit the proliferation,mi-gration and invasion ability of colorectal cancer SW480 cells via UBA2/PTEN/PI3K/Akt signaling pathway.
vitexicarpinSW480 cellsubiquitin-like modification-activating enzyme 2phosphatase and tensin hom-ologsphosphatidylinositol 3-kinaseprotein kinase B
万方数据
维普
