Tanshinone Ⅱ alleviates keen osteoarthritis via NF-κB signaling pathway
Objective It is to explore the mechanism of tanshinone ⅡA in the treatment of knee osteoarthritis based on the nuclear factor κB(NF-κB)pathway.Methods Fifty-two male SD rats were taken,in which 8 rats were randomly se-lected as the sham operation group,and the remaining rats were modeled by modified Hulth method to establish osteoarthri-tis models.The 40 rats that were successfully modeled in 2 weeks after surgery were randomly divided into model group,sodium hyaluronate group,low,medium and high dose groups of tanshinone ⅡA,with 8 rats in each group.The tanshi-none ⅡA groups were given 0.09 mL of sodium tanshinone ⅡA sulfonate injection containing the corresponding dose of raw drug(2.5mg,5mg,10 mg)by joint cavity injection,and the model group and the sham operation group were given equal amount of saline by joint cavity injection,all once per week.After 5 weeks of continuous treatment,the serum levels of in-terleukin-6(IL-6),interleukin-1β(IL-1β),and tumor necrosis factor-α(TNF-α)were detected by ELISA;the degree of osteoarthritic lesions and the contents of collagen fiber were observed by HE staining and Senna O solid green staining,and the degree of cartilage degradation was quantified by Mankin scores;the protein expressions of NF-κB p65,p-NF-κB p65,IκBα and Col Ⅱ in the cartilage tissues of knee joints were detected by Western blot method,and the mRNA expres-sions of NF-κB p65,IκBα and Col Ⅱ in the cartilage tissues of knee joints were detected by qRT-PCR method.Results Compared with the sham operation group,the cartilage damage of rats in the model group was severe,and the serum levels of IL-6,IL-1β,TNF-α,relative protein expressions of IκBα,Col Ⅱ,Aggrecan in cartilage tissues,and relative mRNA expressions of Col Ⅱ,Aggrecan in cartilage tissues were significantly decreased(all P＜0.05),the relative protein ex-pression of p-NF-κB p65 in cartilage tissues,and relative mRNA expressions of NF-κB p65,IκBα in cartilage tissues were significantly increased(all P＜0.05).Compared with the model group,the cartilage damage of rats was relieved in all groups of tanshinone ⅡA and sodium hyaluronate group,the serum levels of IL-6,IL-1β,TNF-α,and relative protein ex-pressions of IκBα,Col Ⅱ,Aggrecan in cartilage tissue,and relative mRNA expressions of Col Ⅱ,Aggrecan in cartilage tissue were significantly increased,while the relative protein expression of p-NF-κB p65 in cartilage tissues,and relative mRNA expressions of NF-κB p65,IκBα in cartilage tissues were significantly decreased in all groups of tanshinone ⅡA and sodium hyaluronate group except for the low dose group of tanshinone ⅡA in which the change of serum level of TNF-α was not significant(all P＜0.05).Conclusion T anshinone ⅡA can delay the development of keen osteoarthritis via in-hibiting NF-κB signaling pathway.
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