Effect of Schisandrin B pretreatment on H2O2-induced pyroptosis and TXNIP/NLRP3 pathway in H9C2 cells
Objective It is to observe the effects of Schisandrin B on the oxidative damage and pyroptosis of H2O2-in-duced H9c2 cardiomyocytes in rats via thioredoxin-interacting protein(TXN1P)/NOD-like receptor protein 3(NLRP3)/Caspase-1 pathway,and to explore its mechanism of myocardial protective effects.Methods H9c2 cardiomyocytes were cul-tured in vitro,and were divided into 4 groups for the experiment:the cells in the normal group were routinely incubated,the cells in the Schisandrin B group were incubated for 24 h with 40 μmol/L Schisandrin B,the cells in the H2O2 group were incubated for 30 min with 1 000 μmol/L H2O2,and the cells in the H2O2+Schisandrin B group were firstly incubated with 40 μmol/L Schisandrin B for 24 h and then with H2O2 for another 30 min.the cell viability was detected by CCK-8 as-say,the content of reactive oxygen species(ROS)was detected by DCFH-DA probe,the contents of lactate dehydrogenase(LDH)in cell culture supernatant,and the contents of malondialdehyde(MDA),superoxide dismutase(SOD),glutathi-one peroxidase(GSH-Px)in the cells were detected by kits,the protein expressions of TXNIP,thioredoxin(Trx),NL-RP3,cleaved Caspase-1,glucocorticoid D(GSDMD-N)in the cells were detected by Western blot,and the levels of inter-leukin-18(IL-18)and interleukin-1 β(IL-1 β)in cell culture supernatant were detected by ELISA.Results Compared with the normal group,the cell viability was significantly decreased in the H2O2 group(P＜0.05),and the contents of ROS,MDA and the relative protein expressions of TXNIP,NLRP3,cleaved Caspase-1,GSDMD-N in the cells and the contents of LDH,IL-1 β and IL-18 in the cell culture supernatant were significantly increased(all P＜0.05),and the con-tents of SOD,GSH-Px content and relative protein expression of Trx in the cells were significantly decreased(all P＜0.05).Compared with the H2O2 group,the cell viability was significantly increased in the H2O2+Schisandrin B group(P＜0.05),and the contents of ROS,MDA and the relative protein expressions of TXNIP,NLRP3,cleaved Caspase-1,GSD-MD-N in the cells and the contents of LDH,IL-1 β and IL-18 in the cell culture supernatant were significantly decreased(all P＜0.05),and the contents of SOD,GSH-Px content and relative protein expression of Trx in the cells were signifi-cantly increased(all P＜0.05).Conclusion Schisandrin B may inhibit cardiomyocyte pyroptosis via affecting the TXNIP/NLRP3/Caspase-1 pathway,thereby attenuating H2O2-induced oxidative damage in H9c2 cells.
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