Effects of decoction for tonifying and replenishing Qi and blood on ovarian granulosa cells apoptosis of controlled ovarian hyperstimulation rats via regulating the expressions of GDF9,c-IAP1/2,JNK1/2,TNFR1/2
Objective It is to observe the effects of post-menstruation proliferation decoction for tonifying and replenis-hing Qi and blood on ovarian granulosa cells apoptosis of controlled ovarian hyperstimulation rats via regulating the expres-sions of growth/differentiation factor 9(GDF9)and cellular inhibitors of apoptosis proteins 1/2(c-IAP1/2),c-Jun N-ter-minal kinase 1/2(JNK1/2),TNF receptor 1/2(TNFR1/2)based on p38MAPK/NF-κB signaling pathway.Methods Twelve female rats were taken and randomly divided into controlled ovarian hyperstimulation+herbal medicine group(6 rats were given post-menstruation proliferation decoction 4.5 g/kg by gavage)and controlled ovarian hyperstimulation group(6 rats were given equal volume of saline by gavage),and after subsequent intraperitoneal injections of triptorelin acetate,serum gonadotropin,and HCG of pregnant horse,the blood from the abdominal aorta of the two groups were taken to pre-pare medicated serum and blank serum.Another 15 female rats were taken and their ovarian granulosa cells were collected after intraperitoneal injections of triptorelin acetate,serum gonadotropin,and HCG of pregnant horse,and were co-cultured in vitro with blank serum(blank serum group),medicated serum(medicated serum group),medicated serum+GDF9 an-tibody(medicated serum+GDF9 antibody group).The apoptosis rate of ovarian granulosa cells was detected by TUNEL,the mRNA expressions of p38 MAPK,NF-κB,GDF9,c-IAP1,c-IAP2,JNK1,JNK2,TNFR1,and TNFR2 in ovarian granulosa cells were detected by qRT-PCR,and the protein expression of GDF9 in ovarian granulosa cells were detected by Western blot.Results The apoptosis rates of both medicated serum group and medicated serum+GDF9 antibody group were significantly lower than that of blank serum group(both P<0.05),and the medicated serum group was significantly lower than the medicated serum+GDF9 antibody group(P<0.05).The relative expressions of p38 MAPK and NF-κB mRNA in the medicated serum group were significantly lower than those in the blank serum group(both P<0.05).The relative expression of c-IAP1,c-IAP2,GDF9 mRNA and GDF9 protein in the medicated serum group and drug-containing serum+GDF9 antibody group were all significantly higher than those in the blank serum group(all P<0.05),and the medicated serum group was significantly higher than the medicated serum+GDF9 antibody group(all P<0.05);the relative expres-sions of JNK1,JNK2,TNFR1 and TNFR2 mRNA in the medicated serum group and medicated serum+GDF9 antibody group were all significantly lower than those in the blank serum group(all P<0.05),and the medicated serum group was significantly lower than the medicated serum+GDF9 antibody group(all P<0.05).Conclusion Post-menstruation prolif-eration decoction for tonifying and replenishing Qi and blood can up-regulate the expressions of GDF9,c-IAP1,c-IAP2,and down-regulate the expressions of JNK1,JNK2,TNFR1,and TNFR2 in ovarian granulosa cells of controlled ovarian hy-perstimulation rats via regulating p38MAPK/NF-κB signaling pathway.
tonifying and replenishing Qi and bloodGDF9c-IAP1c-IAP2TNFR2
万方数据
维普
