Effects of decoction for fortifying spleen,nourishing health Qi and removing mass on the intestinal flora in patients with advanced gastric cancer of type of spleen and stomach deficiency undergoing chemotherapy
Objective It is to explore the possible mechanisms of decoction for fortifying spleen,nourishing health Qi and removing mass in the assist chemotherapy for gastric cancer based on intestinal microecology.Methods Forty-six pa-tients with advanced gastric cancer of type of spleen and stomach deficiency were randomly divided into control group(23 cases)and experimental group(23 cases),another 30 cases of healthy volunteers in the same period were used as the healthy group.The patients in the control group were given chemotherapy based on oxaliplatin combined with fluorouracil,while the patients in the experimental group were orally given decoction for fortifying spleen,nourishing health Qi and remo-ving mass on the basis of chemotherapy(same as that of the control group),and the two groups both completed 2 cycles of chemotherapy.The KPS scores,TCM syndrome scores of the patients before and after treatment and the occurrence of gas-trointestinal adverse reactions after treatment were compared between the two groups;the fecal samples from the patients of the two groups before and after treatment and from 30 healthy volunteers were collected to analyze the species structure and relative abundance of the intestinal flora by 16SrDNA sequencing technology.Results After treatment,the KPS scores of the patients in the experiment group was significantly higher than that before treatment and in the control groups(all P<0.05),and the TCM syndrome scores were significantly lower than that before treatment and in the control groups(all P<0.05),there was no significant changes in the KPS scores and TCM syndrome of the patients in the control group(all P>0.05).The incidence of post-treatment diarrhea of patients in the experiment group was significantly lower than that in the control group(P<0.05).The differences in the Shannon indexes were not statistically significant among the three groups(all P>0.05).The Chao indexes of the patients in the two groups before and after treatment were significantly higher than that in the healthy group(all P<0.05),but the Chao indexe of the patients in the experiment group was sig-nificantly lower than that in the control group after treatment(P<0.05),the species structure of the bacterial flora of pa-tients in the experimental group was more closer to that in the healthy group compared with the control group after treat-ment.Before treatment,the relative abundances of Fusobacteriota,Desulfovibrio,Fusobacterium,Oscillospiraceae_UCG_002 of patients in the two groups were significantly higher than those in the healthy group(all P<0.05),and those of Eu-bacterium_hallii_group were significantly lower than that in the healthy group(P<0.05).After treatment,the relative a-bundance of Eubacterium_hallii_group in the control group was significantly decreased(P<0.05),and the relative abun-dance of Oscillospiraceae_UCG_002 was significantly increased(P<0.05).After treatment,the relative abundance of Eu-bacterium_hallii_group in the experimental group was significantly increased(P<0.05),while the relative abundances of Fusobacteriota and Fusobacterium were significantly decreased(P<0.05),and there was no statistical difference in the relative abundance of Oscillospiraceae_UCG_002 between before and after treatment(P>0.05).Conclusion Decoction for fortifying spleen,nourishing health Qi and removing mass may play its role in the assist chemotherapy for gastric cancer by regulating the richness of intestinal flora,reversing some intestinal bacterial changes caused by chemotherapy,increasing beneficial bacteria,and reducing opportunistic pathogenic bacteria.
gastric cancerchemotherapydecoction for fortifying spleen,nourishing health Qi and removing massintestinal floraspleen and stomach deficiency
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