心不藏神不寐大鼠海马与心肌组织中凋亡相关蛋白表达及细胞凋亡情况研究
Expression of apoptosis-related proteins and apoptosis in hippocampus and myocardium of rats with insomnia due to heart not storing spirit
胡龙涛 1张星平 1陈旭 2王菲 3邓汉沣3
作者信息
- 1. 新疆医科大学第四临床医学院,新疆 乌鲁木齐 830099
- 2. 新疆医科大学附属中医医院,新疆 乌鲁木齐 830099
- 3. 陕西中医药大学,陕西 咸阳 712000
- 折叠
摘要
目的 探讨心不藏神不寐与海马和心肌组织细胞凋亡的关系.方法 将14 只SPF级雄性SD大鼠随机分为模型组与正常组,每组7 只.模型组大鼠采用咖啡因+对氯苯丙氨酸腹腔注射方法构建心不藏神不寐模型,正常组大鼠给予同等剂量生理盐水腹腔注射.注射10d后处死2 组大鼠,RT-PCR法检测海马和心肌组织中B细胞淋巴瘤-2(Bcl-2)、Bcl-2 相关X蛋白(Bax)和半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)mRNA表达情况;免疫组化染色观察海马和心肌组织中Bcl-2、Bax、Caspase-3 蛋白阳性表达情况;TUNEL染色观察海马与心肌组织细胞情况,并计算凋亡率.结果 模型组大鼠海马组织中Bax、Caspase-3 mRNA相对表达量和心肌组织中Caspase-3 mRNA相对表达量均明显高于正常组(P均<0.05),海马组织和心肌组织中Bcl-2 mRNA相对表达量均明显低于正常组(P<0.05);模型组大鼠海马和心肌组织细胞均出现凋亡,细胞凋亡率均明显高于正常组(P均<0.05).结论 心不藏神不寐大鼠海马与心肌组织细胞发生明显凋亡,心不藏神不寐的发生发展与海马和心肌组织细胞的过度凋亡有关.
Abstract
Objective It is to explore the relationship between insomnia due to heart not storing spirit and apoptosis in hippocampus and myocardium.Methods Fourteen SPF male SD rats were randomly divided into model group and normal group,with 7 rats in each group.The rats in the model group were given caffeine+p-chlorophenylalanine(PCPA)by in-traperitoneal injection to establish models of insomnia due to heart not storing spirit,and the rats in the normal group were given the same dose of normal saline by intraperitoneal injection.After 10 days of injection,the rats in the 2 groups were executed,and the mRNA expressions of B-cell lymphoma-2(Bcl-2),Bcl-2-associated X protein(Bax),and cysteine as-partate protease-3(Caspase-3)in the hippocampus and myocardial tissues were detected by RT-PCR;the positive expres-sions of Bcl-2,Bax,and Caspase-3 proteins in the hippocampus and myocardial tissues were observed by immunohisto-chemical staining;the cells in the hippocampus and myocardial tissues were observed by TUNEL staining,and the apopto-sis rate was calculated.Results The relative expressions of Bax and Caspase-3 mRNA in hippocampal tissue and Caspase-3 mRNA in myocardial tissue of the rats in the model group were significantly higher than those in the normal group(all P<0.05),and the relative expressions of Bcl-2 mRNA in hippocampal and myocardial tissues were significantly lower than those in the normal group(all P<0.05).Apoptosis was observed in both hippocampal and myocardial tissue cells of rats in the model group,and the rates of apoptosis were all significantly higher than those in the normal group(both P<0.05).Conclusion Significant apoptosis occurred in the hippocampus and myocardial tissue of rats with insomnia due to heart not storing spirit,and the development of insomnia due to heart not storing spirit was associated with excessive apoptosis of the hippocampus and myocardial tissue.
关键词
不寐/心不藏神/大鼠/海马组织/心肌组织/凋亡Key words
insomnia/heart not storing spirit/apoptosis/hippocampus/myocardium引用本文复制引用
基金项目
国家自然科学基金资助项目(82160873)
国家自然科学基金资助项目(81960837)
国家自然科学基金资助项目(81560762)
国家自然科学基金资助项目(81260526)
新疆自然科学基金重点项目(2022D01D48)
全国名老中医药专家传承工作室(国中医药人教函[2022]75号)
国家中管局高水平中医药重点学科建设项目(国中医药人教函[2023]85号)
新疆"十四五"中医学重点学科(新教函[2022]112号)
出版年
2024
NETL
NSTL
万方数据