龙柴降血方对原发性血小板增多症患者凝血功能及血小板活化蛋白的影响
Effects of Longchai decoction for regulating platelet on coagulation function and platelet activating protein in patients with essential thrombocythemia
郑茂凤 1李芋锦 2杨二鹏 3李雨蒙 4孙妍 1陈科 5陈卓 5吕妍 5王明镜 5胡晓梅6
作者信息
- 1. 北京中医药大学研究生院,北京 100029;中国中医科学院西苑医院,北京 100091
- 2. 云南省第一人民医院,云南昆明 650032
- 3. 清华大学附属北京清华长庚医院,北京 102218
- 4. 中国中医科学院西苑医院,北京 100091;中国中医科学院研究生院,北京 100700
- 5. 中国中医科学院西苑医院,北京 100091
- 6. 中国中医科学院西苑医院,北京 100091;中国中医科学院西苑医院苏州医院(苏州市中医医院),江苏 苏州 215003
- 折叠
摘要
目的 观察龙柴降血方对原发性血小板增多症(ET)患者凝血功能及血小板活化蛋白的影响.方法 选取 2020 年 1 月—2021 年 10 月中国中医科学院西苑医院收治的ET患者 22例,给予龙柴降血方治疗4 个月,观察治疗前后血栓弹力图指标[凝血时间(R)、凝血速率(K)、纤维蛋白功能(Angle)、血栓最大振幅(MA)、血栓最大振幅时间(TMA)、即时振幅(A)、30 min振幅(A30)、从血样开始检测到曲线分叉所需时间(SP)、血凝块溶解时间(CLT)]、高凝状态发生率和积分变化.选取其中15 例ET患者,采用平行反应监测(PRM)技术检测患者治疗前后血小板活化蛋白[血小板膜糖蛋白Ibα(GPIbα)、糖蛋白VI(GPVI)、选择素-P(SELP)]表达水平,分析比较血小板聚集改善患者和血小板聚集增强患者上述各蛋白表达水平的差异;取 5 例健康者和 10 例ET患者治疗前后血清,采用Western blot法检测血清GPIbα表达情况;分析15 例ET患者MA与血清GPIbα表达水平的相关性.结果 与治疗前比较,龙柴降血方治疗后MA、CLT、高凝状态发生率、高凝状态积分和血清GPIbα、GPVI表达水平均明显降低(P均<0.05),血清SELP表达水平无明显变化(P>0.05).治疗后血小板聚集改善患者血清GPIbα表达水平明显低于血小板聚集增强患者(P<0.05),GPVI表达水平无明显变化(P>0.05).与健康人相比,10 例ET患者治疗前血清GPIbα相对表达量明显增高(P<0.05);10 例ET患者治疗后血清GPIbα相对表达量较治疗前明显降低(P<0.05).龙柴降血方治疗后MA下降与GPIbα 表达下调呈正相关(r=0.698 6,P<0.05).结论 龙柴降血方能够抑制ET患者血小板聚集,改善高凝状态,其机制可能与抑制GPIbα蛋白介导的血小板活化信号通路有关.
Abstract
Objective It is to observe the effects of Longchai decoction for regulating platelet on coagulation function and platelet activating protein in patients with essential thrombocythemia(ET).Methods Twenty-two ET patients treated in Xiyuan Hospital of China Academy of Chinese Medical Science from January 2020 to October 2021 were selected and treated with Longchai decoction for regulating platelet for 4 months,the indexes of thromboela-stogram[reaction time(R),kinetics time(K),kinetics of clot development(Angle),maximal amplitude(MA),time to MA(TMA),amplitude(A),30 min amplitude(A30),SP,clot lysis time(CLT)],incidence and scores of hypercoagulable state before and af-ter the treatment were observed.Fifteen of the ET patients were selected,and their expression levels of platelet activation proteins[glycoprotein Ibα(GPIbα),glycoprotein VI(GPVI),and selectin-P(SELP)]before and after treatment were detected by the parallel response monitoring(PRM)technique to analyze and compare the differences in the expression lev-els of the above proteins between the patients with improved platelet aggregation and the patients with enhanced platelet ag-gregation;the serum of 5 healthy patients and 10 ET patients were taken before and after treatment to detect the expression of serum GPIbα by Western blot method;the correlation between MA and the expression level of serum GPIbα in 15 ET pa-tients was analyzed.Results Compared with before treatment,the MA,CLT,incidence and scores of hypercoagulable state,and expression levels of serum GPIbα and GPVI were significantly reduced after treatment with Longchai decoction for regulating platelet(all P<0.05),and there was no significant difference in the expression level of serum SELP(P>0.05).The expression level of serum GPIbα was significantly lower in patients with improved platelet aggregation than that in patients with enhanced platelet aggregation after treatment(P<0.05),but there was no significant change in the ex-pression level of GPVI(P>0.05).Compared with healthy people,the relative expression of serum GPIbα was significant-ly higher in 10 ET patients before treatment(P<0.05);the relative expression of serum GPIbα was significantly de-creased in 10 ET patients after treatment compared with before treatment(P<0.05).There was a positive correlation be-tween the decrease of MA and the down-regulation of GPIbα expression after treatment with Longchai decoction for regula-ting platelet(r=0.6986,P<0.05).Conclusion Longchai decoction for regulating platelet can inhibit platelet aggrega-tion and improve hypercoagulability in patients with ET,and its mechanism may be related to the inhibition of GPIbα pro-tein-mediated platelet activation signaling pathway.
关键词
原发性血小板增多症/龙柴降血方/血栓最大振幅/血小板聚集/血小板活化/血小板膜糖蛋白IbαKey words
essential thrombocythemia/Longchai decoction for regulating platelet/thrombus maximum amplitude/platelet aggregation/platelet activation/platelet membrane glycoprotein Ibα引用本文复制引用
基金项目
国家自然科学基金面上项目(82174360)
国家自然科学基金青年科学基金项目(82304945)
国家自然科学基金青年科学基金项目(82004187)
中国中医科学院科技创新工程重大攻关项目(CI2021A01702)
中国中医科学院科技创新工程重大攻关项目(CI2021A01707)
中国中医科学院基本科研业务费新入职科研人员启动资金专项(ZZ16-XRZ-003)
出版年
2024
NETL
NSTL
万方数据