Effects of Qinhuayin on the tryptophan metabolism pathway and small intestinal pathology in rats with depression of syndrome of spleen-stomach damp-heat
Objective It is to investigate the effects of Qinhuayin on the tryptophan metabolism pathway and small in-testinal pathology in rats with depression of syndrome of spleen-stomach damp-heat.Methods Thirty SD male rats were taken,in which 5 rats were randomly selected as the control group,and the remaining rats were treated with high-fat and high-sugar diet and chronic mild unpredictable stress for 28d to establish models of depression of syndrome of spleen-stom-ach damp-heat.The successfully modeled rats were randomly divided into 6 rats in the model group,6 rats in the Qinhuay-in group,6 rats in the sertraline group and 7 rats in the Qinhuayin+sertraline group.The Qinghuayin group was given Qin-ghuayin 27.5 g/kg by gavage,the sertraline group was given sertraline 5.21 mg/kg by gavage,and the Qinghuayin+ser-traline group was given Qinghuayin and sertraline at intervals of 1 h by gavage,while the model group and control group were given equal amounts of normal saline by gavage,all once daily,continuously treated for 21 days.At the end of the ex-periment,the behavioral conditions of the rats were assessed by open field test;the serum levels of tryptophan,5-hydroxytryptamine(5-HT),and tryptophan were detected by ELISA,and tryptophan/tryptophan and 5-HT/tryptophan ra-tios were calculated;the histopathological morphology of the small intestine were observed by HE staining.Results At the end of the experiment,the rats in the model group were obviously depressive,and their numbers of crossing periphery grid,standing upright were all significantly less than those in the control group(all P<0.05),and the depression of rats in the treatment groups was improved,in which the numbers of crossing center grid of rats in the Qinghuayin group and Qinghuay-in+sertraline group were significantly higher than that in the model group(all P<0.05),the number of crossing peripher-y grid of rats in the Qinghuayin group was significantly higher than that in the Qinghuayin+sertraline group(P<0.05).The serum level of 5-HT,tryptamine/tryptophan ratio,and5-HT/tryptophan ratio in the control group were all significantly lower(all P<0.05),while the serum level of tryptophan was significantly higher in the model group(P<0.05),there was no significant difference in the serum level of tryptamine between the two groups(P>0.05);interstitial lymphocytes in the mucosal lamina propria of small intestinal tissues were aggregated,and lymphoid nodules were formed in the model group.Compared with the model group,the serum level of 5-HT and 5-HT/tryptophan ratio were significantly higher in the Qinghuayin group and Qinghuayin+sertraline group(all P<0.05),while the serum level of tryptamine,tryptamine/trypto-phan ratio in the Qinghuayin group and Qinghuayin+sertraline group and serum level of tryptophan in the Qinghuayin+ser-traline group were significantly lower(all P<0.05);the serum level of 5-HT,tryptamine/tryptophan ratio,and 5-HT/tryptophan ratio in the sertraline group were all significantly higher(P<0.05),while the serum level of tryptophan level was significantly lower(P<0.05),and there was no significant difference in serum level of tryptamine between the two groups(P>0.05);the infiltration of inflammatory cell in small intestinal tissues of the treatment groups was significantly reduced,of which the ratio of the villi length to the crypts depth in the Qinghuayin+sertraline group became larger.Com-pared with the sertraline group,the serum level of tryptophan was significantly higher(P<0.05),and the serum level of tryptamine,tryptamine/tryptophan ratio,and 5-HT/tryptophan ratio were significantly lower in the Qinghuayin+sertraline group(all P<0.05).Conclusion Qinghuayin can improve the depressive symptoms and reduce the pathological damage of small intestine in rats with depression of syndrome of spleen-stomach damp-heat via regulating the metabolic process of tryp-tophan and increasing the serum level of 5-HT,and its combined application with sertraline can increase the serum level of tryptophan,decrease the level of tryptophan and its ratio to tryptophan,thus to increase the utilization rate of tryptophan,and effectively improve the digestive and absorptive functions of small intestine.
depressionQinghuayinsyndrome of spleen-stomach damp-heattryptophan5-HTtryptaminesmall intestine
万方数据
维普
