Objective:To analyze the changes in cellular composition in the heart tissue of wild-type C57B/L6 mice before and after Angiotensin Ⅱ(Ang Ⅱ)infusion using single-cell sequencing technology,particularly focusing on the changes in cardiac fibroblast subpopulations,with the aim of investigating the characteristics of fibroblast alterations during the hypertensive cardiac remodeling process induced by AngⅡ infusion.Method:Heart tissue from mice was collected before(control group)and one week after micro-pump infusion of Ang Ⅱ for single-cell sequencing.The sequencing data were subjected to quality control,normalization,dimension reduction,tSNE clustering,cell type annotation and proportion analysis,followed by functional enrichment analysis.Results:Cells in the mouse heart tissue were classified into seven groups based on characteristic genes,primarily endothelial cells,fibroblasts,and macrophages.After Ang Ⅱ infusion,there was an increase in the proportion of fibroblasts(13.7％vs.24.3％)and macrophages(7.7％vs.22.1％),and a decrease in endothelial cells(72.6％vs.47.6％).Cardiac fibroblasts could be divided into six subgroups,each playing roles in promoting extracellular matrix formation,cell adhesion,and regulating angiogenesis.One week after Ang Ⅱ infusion,there was an increased proportion of fibroblast subgroups involved in inflammatory response and regulation of blood vessel formation.Conclusions:Ang Ⅱ infusion led to an increase in the proportion of fibroblasts and macrophages in cardiac tissue.Among the fibroblasts,subgroups that promote inflammatory responses and regulate blood vessel formation were predominant.
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