Objective:To clarify the role of neutrophil elastase and proteinase 3(NE/PR3)in the early stages of myocardial infarction injury.Methods:ELISA kits were used to detect NE/PR3 levels in peripheral blood of patients with acute infarction and healthy controls;an infarction model was constructed based on coronary artery left anterior descending ligation,and the expression of NE/PR3 in cardiac tissues were detected at 0,1,3,and 7 days after the infarction by WB and RT-QPCR;the effects of knockdown of NE/PR3 were clarified by cardiac function tests performed by small-animal cardiac echocardiographs,immunohistochemical staining,and changes in fibrosis-associated protein(α-SMA)detected by Western blot.Results:The levels of NE/PR3 and the percentage of neutrophils in peripheral blood were significantly higher in patients with myocardial infarction;the expression was significantly higher in cardiac tissues of infarcted mice and was most pronounced mainly at 1 day post-infarction;and NE/PR3-deficient mice showed increased fibrosis in the early infarction period,improved survival in post-infarcted mice,reduced the rate of early cardiac rupture,and improved cardiac function in the early period of injury.Conclusions:Knockdown of neutrophil elastase NE/PR3 promotes cardiac repair by facilitating the conversion of fibroblasts to myofibroblasts thereby reducing the occurrence of dangerous events such as early cardiac rupture and cardiac injury.
维普
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