Objective:To compare and analyze the difference in pain level of different puncture sites through distal transradial access(dTRA)and trans femoral access(TFA)during nerve intervention.Methods:This was a single-center,prospective cohort study.A total of 145 patients,112 males and 33 females,with a mean age of(64.1±8.4)years,were enrolled in the Department of Cerebrovascular Disease,Beijing Anzhen Hospital,from August 2022 to April 2023 for cerebrovascular angiography or neurointerventional therapy.Patients were divided into dTRA group(40 cases)and TFA group(105 cases)according to different types of puncture.The risk factors,laboratory indices,numerical rating scale(NRS),and perioperative adverse events of the two groups were compared for statistical analysis.The univariate and multivariate Logistic regression analysis to further analyze the influencing factors of pain level.Results:The comparison between the dTRA group and the TFA group showed that the proportion of patients with hypertension(81.9%vs.57.5%)and hyperlipidemia(70.5%vs.47.5%)was higher in the TFA group,and there were no significant differences in other risk factors and laboratory indicators between the two groups.The NRS scores of the dTRA group compared with the TFA group showed that the ratio of dTRA/TFA without pain(grade 0)was 67.5%/39%,the mild pain of dTRA(grade 1-3)was significantly lower than that of the TFA group(27.5%vs.44.8%),while the TFA ratio of moderate pain(grade 4-6)was 16.2%.The dTRA group accounted for only 5%.Postoperative pain in dTRA group was significantly lower than that in TFA group,and there was a statistically significant difference between the two groups.Multivariate Logistic regression analysis showed that female and diabetes were the risk factors for puncture pain level.Conclusions:The pain level of dTRA in patients undergoing cerebrovascular angiography or neurointerventional therapy is lower than that of traditional TFA.Female and diabetes were the risk factors for puncture pain level via Logistic regression analysis.