Effects of Oral Administration with Different Doses of Formalin on the Rumen Microbiocoenosis in Sheep
In this study,the effect of the formalin at the beneficial (1.0 mL/kg daily diet)and adverse (3.0 and 4.0 mL/kg)doses on rumen microbiocoenosis was studied.Four small Tail Han-sheep ram with the body weight of 35 kg,1.5-year-old,and with the rumen syrinx,were used with the 4×4 Latin square,fed a diet of concentration (50 ％) and forage (50 ％).The results showed that when the sheep oral administration of formalin with the concentration at 0,1.0,3.0 and 4.0 mL/kg diet,the total number of rumen bacteria was (120.69 ×109 ±17.49 ×109),(191.11×109±20.27×109) (P＜0.01),(94.56 ×109 ±13.01× 109) (P ＜0.05) and (79.60× 109± 12.89× 109) (P ＜0.01)CFU/mL respectively;the total number of rumen protozoa was (9.85× 105 ±0.66×105),(5.02× 105±0.56×105) (P＜0.01),(1.70× 105±0.36×105) (P ＜0.01)and (1.13 × 105 ±0.36 × 105) (P ＜0.01)CFU/mL,respectively;the average concentration of rumen ammonia nitrogen was (25.84±6.65),(21.57±6.07) (P ＜0.01),(15.54±4.41) (P ＜＜0.01),(8.91±3.52) (P ＜0.01) mg/100mL,respectively; the voluntary intake of DM was (2078.4 ± 107.0),(2284.4±55.6) (P ＜0.01),(1965.8±53.4) (P ＜0.01),(1854.5±44.9) (P ＜0.01)g/d respectively.This study showed that oral administration of formalin decreased the number of protozoa,the ammonia nitrogen concentration decreased,but at beneficial dose,the total rumen bacterial and voluntary intake were increased,and at adverse dose,they were decreased.It is concluded that the increase or decrease of rumen bacteria is decisive factor for determining if the oral administration of formalin is beneficial or adverse.
NETL
NSTL
万方数据
维普
