In order to explore the differences of pollen germination and the pollen tube growth of Malus sieversii,this paper used different S-genotypes of Malus sieversii plants as testing materials,by the way of field experiment of pollination to investigate single flower pollen and the hybrid fruit-setting rates of different S genotype combinations,and used the way of style fluorescent microscopy to research the affinitive degree of different S-genotype pollination combinations.The results shows that:the fruit-setting rate of the same S-genotype pollination combination S1S6 × S1S6 was the lowest(2.67 %),the pollen tube germinated late (8 hours after pollination),and grew slower,a few pollen tubes arrived style at base after 48 hours,the majority of pollen tubes arrived at 1/2-2/3 style and stopped to grow,so the degree of self-incompatibility was higher.The pollination combination S6S33 × S1S6 of half-similarity S-genotype had the higher fruit setting rate(8.09 %),the pollen tube germinated late(a large amount germinated at 8-10 hours after pollination),and the speed of growth was moderate,80% of the pollen tubes arrived at style base for 48 hours after pollination,so the degree of affinity was lower.The fruit-setting rate of the different S-genotype pollination combination S21S21 ×S1S6 was the highest (21.27%),the pollen tubes geminated early(a large a mount germinated for 4-8 hours after pollination),all the pollen tubes arrived at style base for 96 hours after pollination,so the degree of affinity was higher.