首页|新疆野苹果不同S基因型间杂交亲和性差异比较

新疆野苹果不同S基因型间杂交亲和性差异比较

Comparison between Different S-genotypes Cross-compatibility and Differences of Malus sieversii

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以不同S基因型的新疆野苹果植株为试验材料,通过田间授粉试验调查了单花花粉量及不同组合S基因型杂交的坐果率,利用花柱荧光显微观察的方法研究了不同S基因型授粉组合的亲和程度.结果表明,相同S基因型授粉组合S1S6×S1S6坐果率最低(2.67%),花粉管萌发较晚(授粉后8h),且生长速度较慢,48 h后有少量花粉管到达花柱基部,大部分花粉管在花柱1/2~2/3处停止生长,自交不亲和程度较强;部分相同S基因型授粉组合S6S33×S1S6坐果率较高(8.09%),花粉管萌发较晚(授粉后8~10 h大量萌发),生长速度适中,48 h后80%花粉管到达花柱基部,亲和程度较弱;不同S基因型授粉组合S21S21×S1S6坐果率高(21.27%),花粉管萌发早(授粉后4~8 h大量萌发),96 h后全部花粉管到达花柱基部,亲和程度较强.
In order to explore the differences of pollen germination and the pollen tube growth of Malus sieversii,this paper used different S-genotypes of Malus sieversii plants as testing materials,by the way of field experiment of pollination to investigate single flower pollen and the hybrid fruit-setting rates of different S genotype combinations,and used the way of style fluorescent microscopy to research the affinitive degree of different S-genotype pollination combinations.The results shows that:the fruit-setting rate of the same S-genotype pollination combination S1S6 × S1S6 was the lowest(2.67 %),the pollen tube germinated late (8 hours after pollination),and grew slower,a few pollen tubes arrived style at base after 48 hours,the majority of pollen tubes arrived at 1/2-2/3 style and stopped to grow,so the degree of self-incompatibility was higher.The pollination combination S6S33 × S1S6 of half-similarity S-genotype had the higher fruit setting rate(8.09 %),the pollen tube germinated late(a large amount germinated at 8-10 hours after pollination),and the speed of growth was moderate,80% of the pollen tubes arrived at style base for 48 hours after pollination,so the degree of affinity was lower.The fruit-setting rate of the different S-genotype pollination combination S21S21 ×S1S6 was the highest (21.27%),the pollen tubes geminated early(a large a mount germinated for 4-8 hours after pollination),all the pollen tubes arrived at style base for 96 hours after pollination,so the degree of affinity was higher.

Malus sieversiiS-genotypepollen germinationpollen tube growth

王云、廖康、杨新峰、邱晨、秦伟

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新疆农业大学特色果树研究中心,乌鲁木齐830052

新疆霍城县大西沟野生樱桃李自然保护区管理站,霍城835200

新疆野苹果 S基因型 花粉原位萌发 花粉管生长

新疆维吾尔自治区自然科学基金

2011211B15

2013

新疆农业大学学报
新疆农业大学

新疆农业大学学报

CSTPCD
影响因子:0.599
ISSN:1007-8614
年,卷(期):2013.36(1)
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