Research on Germination in Vitro Pollen and Tube Growth Dynamics of 4 Apricot Cultivars
4 apricot varieties were cultured in vitro,on the medium the suitable concentrations of sucrose,boric acid for different varieties pollen germination,the dynamic of pollen germination and pollen tube growth of different varieties were observed within 24.0 h after cultured in vitro.The results showed that pollen germination rate increased first and decreased afterwards with the sucrose concentration increasing,the optimum sucrose concentration for Qiaoerpang pollen germination was 15％,those of Dayoujia,Luopuhongdaike and Teerwanyulvke all were 20％.The optimum concentration of boric acid for Qiaoerpang,Dayoujia and Teerwanyulvke germination were 0.01 ％,and then the higher concentration would inhibit the pollen germination,while pollen germination rate of Luopuhongdaike increased first and then decreased with the rise of boric acid concentration.Which boric acid concentration was 0.05 ％ it had the highest germination rate.Among 4 varieties,pollen of Qiaoerpang germinated was in large quantities within 6.0 h after cultured,and rapid growth of pollen tubes lasted to 8.0 h,a large number of pollens germination of Dayoujia duringl.0-10.0h,whilefastgrowingphaseofitspollentubeswereinl.0-6.0h,alargenumberof Luopuhongdaikepollens germinated within 8.0 h,but its pollen tubes grew rapidly only in 4.0 h,the peak of pollen germination and pollen tube growth of Teerwanyulvke both were within 6.0 h after cultured in vitro.In different periods during cultured in vitro there were significant positive correlations between pollen germination rate and pollen tube length of differend varieties.Except Teerwanyulvke,germination rate of pollen of Dayoujia,Qiaoerpang,and Luopuhongdaike were decreased successively,but the sequencing of pollen tube length was exactly the opposite.Pollen germination of different apricot varieties in vitro re quired suitable concentration of sucrose,boric acid,a large number of pollens germinated,and pollen tube rapidly grew in the earlier stage of culture process,and in the late stage of culture process,the pollen germinated and pollen tubes grew both slowly even stagnated,there were differences in pollen germination and growth rate of the different varieties.
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万方数据
维普
