Analysis of Genetic Diversity and Genetic Relationship of Wild Apricot Germplasm in Xinjiang by ISSR Markers
ISSR molecular marker technology was used to amplify 3 populations of wild apricot resources in Yili area,Xinjiang,135 wild apricot samples in order to discuss genetic diversity and genetic relationships of wild apricot germplasm resources in Xinjiang.The results showed that 465 polymorphism bands were amplified with 22 primers by the ISSR-PCR amplification,the percentage of polymorphism loci was 99.49%.Analysis from the species level showed that percentage of polymorphic loci,observed number of al-leles,effective number of alleles,Nei’s genetic diversity and Shannon information index were 99.57%, 1.9957,1.3879,0.2449 and 0.3874 respectively;Analysis from the population levels showed that the aver-age values of percentage of the polymorphic loci,observed number of alleles,effective number of alleles, Nei’s genetic diversity and Shannon information index were 76.80%,1.7680,1.3463,0.2120 and 0.3287 re-spectively.Huocheng ranks first among the different index of three populations,Xinyuan population got the minimum,Gongliu population was in the middle.The genetic variation of the wild apricot resources in Xin-jiang mainly existed in the intra-population (84.85%),and a few existed in the inter-population (15.15%). The genetic relationship of Houcheng population and Xinyuan population was the closest,the genetic varia-tion degree was the smallest;But the genetic relationship between Xinyuan population and Gongliu popula-tion was the farthest,the genetic variation degree was greatest.
wild apricot in Xinjianggermplasm resourcesgenetic diversitygenetic relationshipISSR