首页|响应面法优化半仿生乙醇提取刺山柑果实黄酮工艺

响应面法优化半仿生乙醇提取刺山柑果实黄酮工艺

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利用半仿生醇提法结合响应面法对刺山柑果实中黄酮提取工艺优化.以刺山柑果实黄酮提取量为指标,分析乙醇浓度、提取温度、提取时间、液料比对刺山柑果实黄酮提取量的影响.在单因素试验的基础上用响应面法进行优化,获得半仿生醇提法提取刺山柑果实黄酮的最佳工艺条件为:液料比28:1,提取温度74℃,提取时间71 min,分别用含69%乙醇的3种pH值(2.0、7.5、8.3)提取液对刺山柑果实提取3次,合并3次提取产物.在此工艺条件下,1 g刺山柑果实中黄酮的提取量为24.95 mg.半仿生提取法适用于刺山柑果实黄酮的提取,提取率较高.
Optimization of Semi-bionic Ethanol Extraction Technology of Flavonoids from Capparis spinosa L.by Response Surface Methodology
To optimize the extraction technology of flavonoids in the fruit of Capparis spinosa L.by semi-bimodal alcohol extraction and response surface methodology.The effects of ethanol concentration, extraction temperature,extraction time and liquid ratio on the extraction of flavonoids from citrus reticula-ta were studied.On the basis of single factor experiment,response surface method was used to optimize the extraction of flavonoids from citrus fruit by semi-bionic alcohol extraction.The results showed that the op-timum conditions were as follows:the ratio of liquid to material was 28: 1,and the extraction temperature was 74 ℃.The extraction time was 71 min,and the three kinds of pH value of 69% ethanol were used to extract the fruit of Citrus spinosa for 3 times and combined 3 times to extract the products.Under this condition,the extraction amount of flavonoids from 1 g capers fruit was 24.95 mg.The semi-bionic extrac-tion method was suitable for the extraction of flavonoids from citrus fruit with high extraction rate.

Capparis spinosa L.flavonoidssemi-bionic extractionresponse surface

包晓玮、任薇、张亚涛、曾兰君、魏晨业

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新疆农业大学 食品科学与药学学院,乌鲁木齐 830052

刺山柑 黄酮 半仿生法提取 响应面

新疆维吾尔自治区自然科学基金

2017D01A30

2017

新疆农业大学学报
新疆农业大学

新疆农业大学学报

CSTPCD
影响因子:0.599
ISSN:1007-8614
年,卷(期):2017.40(5)
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