Optimization of Semi-bionic Ethanol Extraction Technology of Flavonoids from Capparis spinosa L.by Response Surface Methodology
To optimize the extraction technology of flavonoids in the fruit of Capparis spinosa L.by semi-bimodal alcohol extraction and response surface methodology.The effects of ethanol concentration, extraction temperature,extraction time and liquid ratio on the extraction of flavonoids from citrus reticula-ta were studied.On the basis of single factor experiment,response surface method was used to optimize the extraction of flavonoids from citrus fruit by semi-bionic alcohol extraction.The results showed that the op-timum conditions were as follows:the ratio of liquid to material was 28: 1,and the extraction temperature was 74 ℃.The extraction time was 71 min,and the three kinds of pH value of 69% ethanol were used to extract the fruit of Citrus spinosa for 3 times and combined 3 times to extract the products.Under this condition,the extraction amount of flavonoids from 1 g capers fruit was 24.95 mg.The semi-bionic extrac-tion method was suitable for the extraction of flavonoids from citrus fruit with high extraction rate.