Functional Analysis of Zinc Finger Protein GhZFP Gene Regulating Verticillium wilt in Cotton
To explore the function of the Gossypium hirsutum C2H2 zinc finger protein family gene GhZFP in the response to Verticillium wilt resistance in cotton.In this research,a C2H2 zinc finger pro-tein-encoding gene,GhZFP,which would respond to the infestation of cotton Verticillium dahliae,was screened and cloned by homologous comparison.Cotton was infested with V.dahliae using the soak-root method,and changes in the relative expression of the GhZFP gene were detected by fluorescence quantifi-cation(qRT-PCR).Then its function was verified using tobacco rattle virus(TRV)virus-induced gene si-lencing(VIGS).The results showed that the relative expression of GhZFP gene was significantly in-creased by 50%at 24 h after V.dahliae treatment compared with the control group.The susceptibility of cotton to Vertillium wilt was elevated after inhibiting the expression of the GhZFP gene using the VIGS technique,and the results of the dissected stem assay showed that plants with silenced genes were browned to a greater extent.From the fluorescence quantification and virus-induced gene silencing results,it can be concluded that the GhZFP gene responds to V.dahliae V991 infestation,and that GhZFP is a positive regulator of cotton's defense against V.dahliae.
万方数据
维普
