Optimization of Tissue Culture in Xinjiang Red Garlic by Using Quadratic Regression Orthogonal Rotation Combination Design
[Objective] This study researched the effects of the plant hormones on Xinjiang red garlic bulb disc adventitious shoots growth in order to provide a theoretical basis for rapid propagation system of Xinjiang white garlic. [Method] The experiment adopted quadratic regression orthogonal rotation combination design and designed different IBA, NAA, 6 - BA level and their combination.[Result] Results showed that the effects on adventitious shoots growth were in the following order; IBA ? NAA ?6 - BA. The effect on IBA was very significant but its high concentration had negative effects, so in further experiments IBA was abolished in order to reduce interaction to Xinjiang red garlic bulb disc adventitious shoots growth effects. 6 -BA had obvious effects on the single - factor with positive effect. [ Conclusion ] The best hormone ration for Xinjiang red garlic bulb disc adventitious shoots growth was: NAA concentration was 4 mg/L, 6 - BA cone ulration was 7.5 mg/L. Under this condition Xinjiang red garlic disc adventitious shoots growth could get an average of 22.92.
国家科技期刊平台
NSTL
万方数据
维普
