Isolation and Identification of Pathogen of Orobanche cumana Stem- rot Disease in Xinjiang
[Objective]Orobancke cumana occurs seriously in Xinjiang. Pathogens were isolated from the disease Orobanche cumana plant and were identified, which can be used as source strains for biological control of Orobanche cumana. [Method]Tissue isolation was used to isolate diseased plant sample, morphology and molecular biology were used to identify Fusarium. [ Result] A total of 587 samples of diseased Orobanche plant were collected and 377 isolates were obtained. According to morphological features of culture, 234 of these, 62.07% of the total isolates, belong to Fusarium; 114 of these, 30. 24% of the total isolates, belong to Rhizotonia; 6 of these, 1.59% of the total isolates, belong to Pythium and 23 of these, 6.46% of the total isolates, belong to others. Three Fusarium species identified were Fusarium oxysporum, F. solani and F. cerealis. [ Conclusion]The main pathogens of Orobanche cumana were Fusarium in Xinjiang, which was a new strain source provided for biological control of Orobanche cumana.