Differential Expression Analysis of Calyx Falling off Korla Fragrant Pears and Their Persistent Related Genes
[ Objective ] The expression of differential gene was studied in calyx falling - off Korla Fragrant Pears and their persistent related genes through the method of DDRT - PCR in order to seek, screen and define the genes and blooming. [Method] Korla Fragrant Pear flower (the second flower and the fourth flower) of the same inflorescence of three anthesis stages (early full bloom, full bloom, the end of bloom) in the same tree were taken as material. Improved DDRT - PCR method was used to investigate the differential display of genes relate to calyx leaving and persistent related genes of Korla Fragrant Pears. [Result] The result showed that the differential display exist in early full bloom stage, 42 different expressed cDNA fragments were obtained and only 7 double - primer bands were amplified. The alignments of nucleotide and amino acid sequences indicated that there were 2 fragments which have high homology that might be closely related to SPL and MYB, the two translation factors important to regulation on plant phytohormone and flowering. The homology was 78% and 86.83% , respectively. [Conclusion] This study had established the system of DDRT - PCR to obtain genes which relate to calyx of leaving and persistent. It laid on a foundation for cloning full length of relative gene,which also makes insurance to the further studying its physiological and biochemical characteristics and functions of thd gene expression product.
国家科技期刊平台
NSTL
万方数据
维普
