Physiological Response of Pepper Seedlings to Low Temperature Stress under Exogenous Substance Treatment
[Objective] This research aims to study the effects of exogenous substances on physiological and biochemical characteristics of pepper seedlings during chilling stress.[Method]The concentration of 0.5 mmol/L spermidine,50 mg/L chitosan,2 mmol/L salicylic acid and 10 mmol/L glycine betaine solution were sprayed on the pepper seedlings respectively,and then content of soluble sugar,proline,soluble protein,malondialdehyde,chlorophyll and activity of SOD,POD were measured and analyzed after 0,2,4,6,8 days under the day temperature/night temperature 10℃/5℃ and recovered 2 days under 25℃/20℃,The effect of exogenous substances on physiological and biochemical characteristics of pepper seedlings during low temperature stress was then discussed in this paper.[Result] The results indicated that appropriate concentration of exogenous spermidine,chitosan,salicylic acid and glycine betaine treatment could reduce MDA content,increase the content of soluble sugar,proline,soluble protein,chlorophyll and enhance the activity of SOD and POD in the leaves of pepper seedling during the low temperature stress.[Conclusion] All of the four kinds of exogenous substances produced positive influence on improving chilling resistance of pepper seedlings,the effect of which on cold resistance was spermidine ＞ chitosan ＞ betaine ＞ salicylic acid.
spermidinechitosansalicylic acidglycine betaineexogenous substancespepperlow temperature stressphysiological response
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