首页|基于SSR标记的葡萄品种(系)遗传多样性分析与指纹图谱构建

基于SSR标记的葡萄品种(系)遗传多样性分析与指纹图谱构建

扫码查看
[目的]对供试葡萄材料进行遗传多样性分析,构建其指纹图谱,为葡萄分类、种质鉴定和制干葡萄定向育种提供科学依据.[方法]利用SSR标记对新疆44个相对适宜制干葡萄(VitisL)品种(系)进行遗传多样性分析及指纹图谱构建.[结果]以52对SSR引物对供试材料的基因组DNA进行PCR扩增,筛选出8对多态性高、谱带清晰的引物.共扩增出190条带,均为多态性条带,多态性百分率为100%.多态性信息含量指数(PIC)变幅为0.686 8~0.964 0,平均为0.908 4.UPGMA聚类分析表明,44份葡萄品种(系)间遗传相似系数的变异范围为0.63~0.92,在遗传相似系数0.654处,可将44份供试材料分为5个类群,在一定程度上反映了品种之间的亲缘关系.利用Vmc9a2.1、UDV-017、UDV-033和UDV-041等4条引物构建了品种DNA指纹图谱,可区分44个供试材料.[结论]SSR标记方法可分析供试材料的亲缘关系,利用筛选出的4种引物可构建其DNA指纹图谱.
Analysis of Genetic Diversity and Construction of DNA Fingerprinting for Vitis Germplasm Based on SSR Markers
[Objective]The project aims to clarify the genetic diversity and establish the fingerprinting among the materials in this study, the present results provide valuable tools for the cultivar classification, germplasm identification and orientational breeding of dried grape.[Method]SSR markers were employed to clarify the genetic diversity and establish the fingerprinting of 44 grape (Vitis L.) genotypes suitable for processing of raisins.[Result]A total of 190 makers were scored from the 8 primers, which were screened out from 52 primers and demonstrated highly reproducible, clear and polymorphie band.190 DNA fragments were generated and all of them were polymorphic bands (the percentage of polymorphic band, PPB =100%).The ranges of PIC at 8 SSR loci of 44 cultivars were 0.686,8-0.964,0, with an average of 0.908 4.The clustering dendrogram was constructed by UPGMA method, the genetic similarity coefficient of the 44 accessions ranged from 0.63 to 0.92;all the materials were divided into 5 major groups while the similarity coefficient was 0.654, and to a certain extent reflect the evolutionary relationship among them.The primer pairs Vmc9a2.1, UDV-017, UDV-033 and UDV-041 were selected to establish the fingerprint of grape, which was able to distinguish all of the 44 accessions.[Conclusion]Using the method of SSR molecular markers could analyze the genetic relationship of the germplasms, and the four kinds of primers which were selected from the study could build its DNA fingerprint.

grapeSSR markersgenetic diversitycluster analysisDNA fingerprinting

郭春苗、李宁、周晓明、樊丁宇、谢辉、张付春、潘明启、卢春生

展开 >

新疆农业科学院园艺作物研究所/农业部新疆地区果树科学观测试验站,乌鲁木齐830091

葡萄 SSR标记 遗传多样性 聚类分析 指纹图谱

新疆农业科学院青年基金现代农业产业技术体系专项资金

xjnky-2013003CARS-30-zp-03

2015

新疆农业科学
新疆农业科学院 新疆农业大学 新疆农学会

新疆农业科学

CSTPCDCSCD北大核心
影响因子:0.698
ISSN:1001-4330
年,卷(期):2015.52(11)
  • 14
  • 15