Transcriptome and metabolome integrated analysis of flavonoids in Xinyu grape peel under different ground mulch 5ypes
[Objective]To clarify the flavonoid related components and synthase genes information of ta-ble grapes under two different ground covering methods of horticultural cloth covering and grass cultivation in the hope of laying the theoretical foundation for proposing a new mode of high efficiency and high quality culti-vation of Xinyu grapes in production.[Methods]In this study,the ground mulched by black geotextile and grass,clean tillage was taken as the control,the cDNA of Xinyu grape peel flavonoids were sequenced and were analyzed by using the bioinformatics methods subsequently,such as sequencing assess and gene function anno-tation,at the same time,the flavonoid metabolites in grape peel were determined by LC-MS/MS.[Results](1)Compared the transcriptome of the black geotextile mulch and grass,587 differentially expressed gene(DEGs)in black geotextile mulch were found,including 317 up-regulated gene and 270 down-regulated genes,177 differentially expressed gene(DEGs)in grass were found,including 56 up-regulated gene and 121 down-regulated genes.(2)GO function annotation gene of black geotextile mulch was divided into 45 func-tion categories,and the GO annotation gene of grass type was divided into 37 function categories,in which many function categories were mainly involved,such as cellular component,molecular function and biological proces-ses.KOG database classified the 175 and 86 DEGs annotated to,respectively,with 22 and 16 functional cate-gories respectively,and 193 DEGs for black geotextile mulch were annotated into KEGG pathway,biosynthesis of secondary metabolites,phenylpropanoid biosynthesis,flavonoid biosynthesis,phenylalanine biosynthesis,cir-cadian rhythm-plant,stilbenoid,diarylheptanoid and gingerol biosynthesis were associated with flavonoid en-richment pathway,61 DEGs for grass were annotated into KEGG pathway,biosynthesis of secondary metabolites was associated with flavonoid enrichment pathway.(3)A total of 60 flavonoids metabolites were detected for Xinyu grape peel and divided into 12 categories,including 7 differential metabolites(2 down-regulated and 5 up-regulated)for black geotextile mulch and 11 differential metabolites(all down-regulated)for grass culti-vation.Flavanols proportion content was the highest,with 52.41％-63.70％,flavanols content were higher for grass cultivation and black geotextile mulch than for the control,with 19.42％and 7.55％.(4)The fla-vonoid biosynthesis related enrichment pathways for black geotextile mulch were mainly concentrated in the fla-vonoid biosynthesis,while the flavonoid biosynthesis related enrichment pathways of grass mulch were mainly concentrated in the secondary metabolites and flavonoid biosynthesis,seven candidate genes were screened in-cluding C4H,CHS,F3H,LAR,HCT,FLS and C12RT1 for black geotextile mulch,five candidate genes were screened including CHS,F3H,F3′5′H,FLS and LAR for grass.[Conclusion]By transcriptome and metabolite analysis,candidate genes for flavonoid biosynthesis of Xinyu grape were screened and analyzed for black geo-textile and grass mulch,grass cultivation was the preferable cultivation method for Xinyu grape in Xinjiang Tur-pan extreme drought area.
万方数据
维普
