Study on establishment and optimization of rapid tissue culture propagation technology of three wine grape cultivars
[Objective]In order to improve the quality of wine grape seedlings and the breeding speed of improved varieties,the tissue culture virus-free and rapid propagation technology system of wine grape varie-ties Dunkelfelder T,Petit Verdot and Sauvignon Blanc was preliminarily established.Which has provided the basis for improving the quality of seedlings of small wine grape varieties and rapid propagation.[Methods]Three one-year axillary buds of wine grape were used as explants,and the orthogonal test design was used to explore the effects of different disinfection methods on the survival rate of explants,and the effects of different concentrations of 6-BA,IBA,NAA and different combinations of media on the germination,proliferation and rooting of axillary buds.[Results]The results showed that the survival rate of axillary buds could be improved and the pollution rate could be reduced by 10％sodium hypochlorite disinfection for 7.5min,75％ethanol dis-infection for 30 s,and 0.1％HgCl2 disinfection for 10min;When the sterilized explants were inoculated into MS medium supplemented with 1.0 mg/L 6-BA and 0.05 mg/L NAA,the germination rate was the highest.When the sterile new buds of the start-up culture were added into the 1/2MS medium supplemented with 1.0 mg/L 6-BA and 0.3 mg/L IBA,the clustered buds had the best proliferation effect.When the single buds af-ter the proliferation culture were put into the 1/2MS rooting medium supplemented with 0.4 mg/L IBA and 0.4 mg/L NAA,the overall quality of rooting of tissue culture seedlings reached the best effect.[Conclusion]The technical system of tissue culture and rapid propagation and virus-free of three wine grape varieties has been preliminarily established.The heat treatment combined with shoot tip for virus-free of tissue culture seedlings was applied in the experiment,and the results showed that this method could remove most of grape vi-ruses.
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