Isolation and screening of cultivable endophytes in sifferent organs of Glycyrrhiza uralensis Fisch and preliminary screening of β-glucosidase producing strains
[Objective]To study the resource status of culturable endophytes from different parts of Gly-cyrrhiza uralensis Fisch Altay region of Xinjiang and to screen high-yield β-glucosidase strains.[Methods]Using Glycyrrhiza uralensis Fisch as the material,the endophytic bacteria in its taproot,fibrous root,stem and leaf were isolated,purified and identified by means of tissue homogenization combined with 16S rDNA and ITS-rDNA molecular biology methods.The β-glucosidase producing strains were screened by aescin medium and β-glucosidase activity determination.[Results]The results showed that 48 endophytes,33 endophytic bacteria and 15 endophytic fungi were isolated from Glycyrrhiza uralensis Fisch.The number of endophytic bacteria isolated from taproots and stems was the largest,and that of endophytic fungi colonized in taproots was the largest.The 33 endophytic bacteria from Glycyrrhiza uralensis Fisch belonged to 8 genera,and the most Ba-cillus were isolated,and the endophytic bacteria in the taproot of Glycyrrhiza uralensis Fisch were the most widely isolated species.The diversity in different parts was as follows:taproot>fibrous root = stem = leaf.15 endophytic fungi from Glycyrrhiza uralensis Fisch belonged to 7 genera,Cladosporium was the dominant ge-nus of endophytic fungi.The diversity of endophytic fungi in different parts was as follows:taproot>stem>leaf>fibrous root.A total of 12 strains had the ability to produce β-glucosidase,among which the most strains isolated from taproots,accounted for 41.7％.[Conclusion]The diversity of taproot organ of cultivable endophytes from Glycyrrhiza uralensis Fisch is rich and there are more β-glucosidase producing strains,which are the dominant part for breeding enzyme-producing strains.
万方数据
维普
