新疆农业科学2024,Vol.61Issue(2) :336-344.DOI:10.6048/j.issn.1001-4330.2024.02.009

NaCl胁迫下外源亚精胺对马铃薯幼苗生理特征的影响

Effect of exogenous spermidine on physiological characteristics of potato seedlings under NaCl stress

刘易 李江涛 江应红 杨茹薇 孙慧 吴燕
新疆农业科学2024,Vol.61Issue(2) :336-344.DOI:10.6048/j.issn.1001-4330.2024.02.009

NaCl胁迫下外源亚精胺对马铃薯幼苗生理特征的影响

Effect of exogenous spermidine on physiological characteristics of potato seedlings under NaCl stress

刘易 1李江涛 1江应红 1杨茹薇 1孙慧 1吴燕1
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作者信息

  • 1. 新疆农业科学院综合试验场,乌鲁木齐 830013
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摘要

[目的]研究NaCl胁迫下外源亚精胺(Spd)对马铃薯幼苗生理特征的影响,为新疆盐渍化区域马铃薯种植提供技术参考.[方法]以晋薯16 号马铃薯幼苗为材料,采用不同浓度NaCl模拟盐胁迫处理,研究外源亚精胺Spd对马铃薯植株生长、叶片抗氧化酶活性、渗透调节物质含量的影响.[结果]盐胁迫抑制了马铃薯植株生长,使得超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)、抗坏血酸过氧化物酶(APX)等酶促类抗氧化酶活性表现为低盐胁迫促进,高盐胁迫抑制;抗坏血酸(ASA)、谷胱甘肽(DSH)等非酶促类抗氧化酶含量均降低;脯氨酸、可溶性糖、氨基酸含量升高;外源亚精胺(Spd)通过提高叶绿素含量、根系活力,调节渗透调节物质含量,增强抗氧化酶活性等方式维持植物体的正常生理代谢功能.[结论]外源亚精胺(Spd)可提升马铃薯耐盐性,缓解盐胁迫对马铃薯植株的抑制作用,其中 0.9 mmol/L Spd(S3)浓度缓解效果最明显.

Abstract

[Objective]To explore the effect of exogenous spermidine on the physiological characteristics of potato seedlings under NaCl stress,so as to provide technical reference for potato planting in salinized areas of Xinjiang.[Methods]The virus-free seedling of Jindu 16 potato was used as the experimental material,and the effects of exogenous Spd on plant growth,antioxidant enzyme activity and osmotic regulator content of potato leaves were studied by simulating salt stress treatment with different concentrations of NaCl.[Results]Salt stress inhibited the growth of potato plants.The activities of antioxidant enzymes such as superoxide dis-mutase(SOD),peroxidase(POD),catalase(CAT)and ascorbate peroxidase(APX)were promoted by low salt stress and inhibited by high salt stress.The contents of non-enzymatic antioxidant enzymes such as ascorbic acid(ASA)and glutathione(DSH)were decreased.The contents of proline,soluble sugar and amino acid in-creased.Exogenous Spd maintained the normal physiological and metabolic function of plants by increasing the content of chlorophyll,root activity,regulating the content of osmotic regulatory substances,and enhancing the activity of antioxidant enzymes.[Conclusion]Exogenous Spd can improve the salt tolerance of potato and alle-viate the inhibitory effect of salt stress on potato plants,and 0.9 mmol/L Spd(S3)concentration has the most obvious alleviating effect.

关键词

马铃薯/NaCl胁迫/亚精胺/幼苗生长/生理生化特征

Key words

potato/NaCl stress/spermidine/seedling growth/physiological and biochemical characteristics

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基金项目

新疆维吾尔自治区重点研发任务专项(2022B02044-2)

新疆维吾尔自治区自然科学基金-面上项目(2020D01A68)

出版年

2024
新疆农业科学
新疆农业科学院 新疆农业大学 新疆农学会

新疆农业科学

CSTPCDCSCD北大核心
影响因子:0.698
ISSN:1001-4330
参考文献量37
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