摘要
[目的]研究60Co-γ射线对不同无花果品种一年生枝条死亡率、抗氧化酶活性等相关理化指标的影响,确定最佳辐射诱变剂量,为利用60Co-γ射线辐射诱变技术提高无花果遗传多样性、创造新种质、培育优良新品种提供科学依据.[方法]以无花果品种布兰瑞克和中华紫果一年生休眠枝为试材,采用60Co-γ射线为辐照源,设置不同剂量0、30、45、60、75 Gy的辐射处理,分析辐照引起枝叶死亡率、酶活性等变化.[结果]随着辐射剂量的增加,2 个无花果品种扦插苗成活率呈下降趋势,株高、茎粗、节间长度、叶片长度、叶片宽度和分枝数指标呈下降趋势,叶片长宽比差异不显著.中华紫果品种的SOD、POD、CAT等酶活性均呈现先增后减的趋势,分别在45、60 Gy剂量时达到峰值,MDA含量呈现一直上升趋势,H2O2含量呈现先增后降趋势.布兰瑞克品种的SOD、POD、CAT酶活性,随着辐射剂量的增加均呈现先降后增趋势.MDA、H2O2 含量基本保持上升趋势.[结论]辐射导致无花果扦插苗幼苗生长缓慢、株高矮化、茎粗变细、叶片变小、分枝减少.中华紫果的半致死辐射剂量为56 Gy,布兰瑞克为70 Gy,布兰瑞克对辐射的耐受性高于中华紫果.
Abstract
[Objective]To explore the influence of 60Co-γ radiation on the mortality rate,antioxidant en-zyme activity and other related physical and chemical indexes of annual cuttings of different fig varieties,and determine the best mutagenic dosage.This study has provided a scientific theoretical basis for the future use of 60Co-γ radiation mutagenesis to improve the genetic diversity of fig,create new germplasm and cultivate new excellent varieties.[Methods]The annual dormant branches of Brunswick and Zhonghuaziguo were used as test materials,and 60Co-γ radiation as the irradiation source,different dosages(0,30,45,60,75 Gy)for radiation treatment were set to observe the changes in the death rate and enzyme activity of branches and leaves caused by irradiation.[Results]With the increase of radiation dosage,the survival rate of cutting seedlings of the two varieties showed a downward trend,and the indexes of plant height,stem diameter,internode length,leaf length,leaf width and branch number showed a downward trend,while the difference of leaf length-width ratio was not significant.The activities of SOD,POD,CAT and other enzymes of Zhonghuaziguo varie-ties showed a trend of increasing first and then decreasing,reaching the peak at the dosage of 45 and 60 Gy,respectively,MDA content showed a trend of increasing all the time,and H2O2 content showed a trend of in-creasing first and then decreasing.The activities of SOD,POD and CAT enzymes of Brunswick varieties de-creased first and then increased with the increase of radiation dose.The content of MDA and H2O2 basically kept rising.[Conclusion]Radiation leads to slow growth of fig cutting seedlings,dwarf plant height,coarse and thin stem and leaves,smaller leaves and fewer branches.It is preliminarily determined that the appropri-ate half lethal dose of Zhonghuaziguo is 56 Gy,and that of Brunswick is 70 Gy.The radiation tolerance of Brunswick is higher than that of Zhonghuaziguo.
基金项目
新疆维吾尔自治区自然科学基金面上项目(2021D01A108)
万方数据