Effects of different feeding time of Monolepta Signata on the composition and content of volatile in maize leaves
[Objective]To analyze the composition and content of maize leaf volatile before and after the damage caused by Monolepta signata in the hope of providing a scientific basis for further exploring the defense mechanism of maize leaf volatile against M.signata.[Methods]In this study,the volatile compounds of maize leaves and M.signata were detected and analyzed by GC-MS at different feeding induction time(6,24,36 and 48 h).[Results]The results showed that 29,30,33,36 and 26 volatile substances,mainly including aldehydes,terpenoids,alcohols and ketones,were detected after 0,6,24,36 and 48 h of feeding induction by the M.signata respectively.Twenty volatiles,including 1-stigmasterene,cis-11-hexadecanal,α-stig-masterene and hexanal,disappeared with the insect after feeding on the damage;42 compounds,such as 2-hexenal,(E)-2-hexenal,α-pinene and β-laurene,were newly collected.The results of principal compo-nent analysis showed that α-pinene,2-hexenal and β-pinene were the main compounds released under 6h and 24h of infestation;hexadecanal,tetradecanal,n-pentadecanal,β-pirolone and(E)-3-hexen-1-ol were the main compounds released at 36 h of infestation;β-red myrcene alcohol,nerolidol,(1R)-α-pi-nene,β-rolene,β-sandalene β-cineole,and humulene were the main compounds released at 48 h of in-sect infestation.[Conclusion]The composition and content of volatile compounds in maize leaves at different feeding times have a significant impact on the M.signata.Under 6 and 24 hours of pest infestation,it mainly releases olefins,while at 36 hours of pest infestation,it mainly releases aldehydes and ketones.After 48 hours of pest infestation,it mainly releases alcohols and olefins.
万方数据
维普
