摘要
[目的]筛选出抑制梨火疫病的药剂,为梨火疫病的绿色安全防控提供技术支持.[方法]采用抑菌圈法筛选22 种抗生素,评价其在库尔勒香梨花器上的安全性.[结果]13 种抗生素对梨火疫病菌有抑制作用,其中在抑菌圈直径达到9mm时,氯霉素①、注射用头孢噻肟钠、注射用头孢曲松钠和诺氟沙星的药剂有效浓度由低至高是5.30、7.60、9.88、11.64 mg/L,使用同等剂量下4 种药剂的抑制作用强于其它药剂;氯霉素①、注射用头孢噻肟钠、注射用头孢曲松钠、罗红霉素分散片、诺氟沙星对库尔勒香梨花器的危害较轻.[结论]氯霉素①、诺氟沙星、注射用头孢曲松钠和注射用头孢噻肟钠可在田间防治梨火疫病上应用.
Abstract
[Objective]This research aims to screen out antibiotics that can effectively control Erwinia amylovora in the hope of providing technical support for the green safety prevention and control of the disease.[Methods]22 kinds of antibacterial agents and preparations were screened in the laboratory using bacterio-static circle,and then the safety on floral organ of Korla Fragrant Pear was evaluated.[Results]13 kinds of antibiotics had good antibacterial effect on Erwinia amylovora,and when the diameter reached 9 mm,the ef-fective concentration of chloramphenicol①,cefotaxime sodium for injection,ceftriaxone sodium for injection and norfloxacin from low to high in order were 5.30,7.60,9.88,11.64 mg/L,indicating that using the same dosage,the inhibitory effect of four antibiotics were stronger than those of other antibiotics;The floral organ of Korla Fragrant Pear suffered less injury when using the chloramphenicol①,cefotaxime sodium for injection,ceftriaxone sodium for injection,roxithromycin dispersible tablet and norfloxacin.[Conclusion]The selected chloramphenicol ①,norfloxacin,ceftriaxone sodium for injection and cefotaxime sodium for injection can be used in the field to control Erwinia amylovora in fields.
基金项目
新疆生产建设兵团财政科技计划(重点领域科技攻关计划项目)(2018AB038)